连翘多胺氧化酶基因家族的鉴定及干旱和盐胁迫下的表达分析  

Identification and expression analysis of Forsythia suspensa PAO gene family under salt and drought stress

作　　者：陈佳茜 郭广洋 谭新杰 吕淑芳[1,2] 胥华伟 原梦[1,2] 邓萍 侯典云 CHEN Jiaxi;GUO Guangyang;TAN Xinjie;LV Shufang;XU Huawei;YUAN Meng;DENG Ping;HOU Dianyun(College of Agriculture,,Henan University of Science and Technology,Luoyang 471023,China;Henan Engineering Research Center for Evaluation and Innovative Utilization of Homology of Medicine and Food,Luoyang 471023,China)

机构地区：[1]河南科技大学农学院,河南洛阳471023 [2]河南省药食兼用资源评价与创新利用工程研究中心,河南洛阳471023

出　　处：《中草药》2024年第9期3077-3084,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(U1404829);中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302);河南省自然科学基金资助项目(202102110156);河南省科技攻关(242102110325);河南省中药材产业科技特派员服务团、河南省中药产业技术体系建设专项资金资助。

摘　　要：目的研究连翘多胺氧化酶(polyamine oxidase,PAO)基因家族在盐和干旱胁迫下的响应特征。方法通过分析连翘转录组数据鉴定出5个连翘PAO基因家族成员,结合蛋白质理化性质和基序分析、蛋白质结构分析、系统进化分析、结构域等方式进行相关性分析,并探究了盐和干旱胁迫下PAO基因的表达水平。结果连翘PAO基因家族氨基酸数介于235~541 aa,相对分子质量在26069~59328,等电点在5.27~5.86;不稳定系数为35.68~44.14,总平均亲水性皆为负值,推测连翘PAO家族蛋白均为不稳定型、酸性、亲水性蛋白,α-螺旋与无规则卷曲为蛋白主要二级结构形式。结论鉴定出的5个连翘PAO基因家族成员均具有特异性表达模式;在盐和干旱胁迫下5个连翘PAO基因家族成员的表达均受到诱导,可能参与连翘的盐胁迫及干旱胁迫响应调控,为深入探讨连翘PAO基因家族的功能奠定了基础。Objective To study the response characteristics of Lianqiao(Forsythia suspensa)polyamine oxidase(FsPAO)gene family under salt and drought stress.Methods Five members of the FsPAO gene family were identified by analyzing the F.suspensa transcriptome data.The correlation analysis was carried out by means of protein physical and chemical properties and motif analysis,protein structure analysis,phylogenetic analysis,and structural domains.The gene expression levels were analyzed under salt and drought stress.Results The amino acid number of the FsPAO gene family ranged from 235 to 541 aa;The molecular weight from 26069 to 59328;The isoelectric point ranged from 5.27 to 5.86;The instability coefficients from 35.68 to 44.14,and the total average hydrophilicity was negative,so it was hypothesized that all the proteins of the FsPAO gene family were unstable,acidic,hydrophilic proteins,withαHelix and irregular coil being the main secondary structural forms of PAO proteins.Conclusion The five identified members of the FsPAO gene family had specific expression patterns,and salt stress and drought stress induced the expression of the five members of the FsPAO gene family,which might be involved in the regulation of F.suspensa in response to salt stress and drought stress.Through this study lays a foundation for in-depth exploration of the function of the FsPAO gene family.

关 键 词：连翘 PAO基因家族 基因表达 胁迫 蛋白质结构分析 

分 类 号：R286.12[医药卫生—中药学]