锦灯笼宿萼中甾体类化学成分与抗炎活性  

Steroid chemical constituents and anti-inflammatory activity in calyx of Physalis alkekengi var.franchetii

作　　者：童正香 刘璟钰 陈鑫 李蓉涛[1] 曾建国 TONG Zhengxiang;LIU Jingyu;CHEN Xin;LI Rongtao;ZENG Jianguo(School of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Hunan Key Laboratory of Traditional Chinese Veterinary Medicine,College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;Shanxi Key Laboratory of Modernization of Chinese Veterinary Medicine,College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]湖南农业大学动物医学院中兽药湖南省重点实验室,湖南长沙410128 [3]山西农业大学动物医学学院中兽医药现代化山西省重点实验室,山西太谷030801

出　　处：《中草药》2024年第10期3230-3237,共8页Chinese Traditional and Herbal Drugs

基　　金：“十四五”国家重点研发计划课题(2022YFD1801103)。

摘　　要：目的研究锦灯笼Physalis alkekengi var.franchetii宿萼95%乙醇提取物的化学成分与抗炎活性评价。方法采用硅胶柱、Sephadex LH-20凝胶柱、HPLC制备等方法进行分离、纯化,通过波谱技术对化合物结构进行鉴定,采用脂多糖(lipopolysaccharide,LPS)诱导的RAW264.7小鼠单核巨噬细胞体外炎症模型进行抗炎活性评价。结果分离得到14个化合物,分别鉴定为7β-ethoxy-25,27-dihydro-3,6-didehydroisophysalin M(1)、异酸浆苦素B(2)、4,7-didehydroneophysalin B(3)、酸浆苦素B(4)、25,27-dihydro-4,7-dedehydro-7-deoxyneophysalin A(5)、酸浆苦素E(6)、酸浆苦素L(7)、酸浆苦素O(8)、酸浆苦素D(9)、ergosta-4,24(28)-diene-3-one(10)、24-ethylcholesta-4,24(28)-dien-3-one(11)、sitosterone(12)、gramisterol(13)、柠黄醇(14);在抗炎活性评价实验中,化合物2~6、8~9对NO生成具有抑制作用。结论化合物1为新化合物,命名为酸浆苦素M1;化合物10~12、14均为首次从该属植物中分离得到;此外,化合物6对NO具有显著抑制作用,半数抑制浓度(median inhibition concentration,IC50)值为15.07μmol/L,与阳性L-NMMA(IC50=36.11μmol/L)相比具有较好的抗炎活性。Objective To study the chemical composition and anti-inflammatory activity of 95%ethanol extract in the calyx of Jindenglong(Physalis alkekengi var.franchetii).Methods These compounds were separated and purified by silica gel column,Sephadex LH-20 gel column and HPLC preparation,and their structures were identified by spectroscopic techniques,the antiinflammatory activities of RAW264.7 mouse mononuclear macrophages induced by LPS were evaluated in vitro.Results Fourteen compounds were isolated and their structures were identified as 7β-ethoxy-25,27-dihydro-3,6-didehydroisophysalin M(1),isophysalin B(2),4,7-didehydroneophysalin B(3),physalin B(4),25,27-dihydro-4,7-dedehydro-7-deoxyneophysalin A(5),physalin E(6),physalin L(7),physalin O(8),physalin D(9),ergosta-4,24(28)-diene-3-one(10),24-ethylcholesta-4,24(28)-dien-3-one(11),sitosterone(12),gramisterol(13),citroflavin(14).In the experiment of anti-inflammatory activities evaluation,compounds 2—6,8—9 had inhibitory effect on NO production.Conclusion Compounds 1 is a new compound named physalin M1.Compounds 10—12,14 were isolated from this plant for the first time.In addition,compound 6 had a significant inhibitory effect on NO,and the IC50 of compound 6 was 15.07μmol/L,compared with positive L-NMMA(IC50=36.11μmol/L),it had better anti-inflammatory activity.

关 键 词：锦灯笼 甾体类化合物 抗炎活性 酸浆苦素M1 酸浆苦素E 酸浆苦素O 柠黄醇 

分 类 号：R284.1[医药卫生—中药学]