自组装球形氧化铈抑制胃癌细胞增殖和迁移基础研究  

Self-Assembled Spherical Cerium Oxide Inhibit Migration and Proliferation of Gastric Cancer

作　　者：关达 张振羽 刘璐[1] 吴迪[1] 宋奇赢 谢天宇 徐旸[2] 王鑫鑫[1] 冯宗玉[2] 黄小卫[2] Guan Da;Zhang Zhenyu;Liu Lu;Wu Di;Song Qiying;Xie Tianyu;Xu Yang;Wang Xinxin;Feng Zongyu;Huang Xiaowei(Department of General Surgery,First Medical Center of PLA General Hospital,Beijing 100853,China;General Research Institute for Nonferrous Metals,Beijing 100088,China)

机构地区：[1]解放军总医院第一医学中心普通外科医学部,北京100853 [2]北京有色金属研究总院,北京100088

出　　处：《稀有金属》2024年第4期517-528,共12页Chinese Journal of Rare Metals

基　　金：北京市科技新星交叉课题基金项目(20220484827)资助。

摘　　要：胃癌(gastric cancer,GC)是全球最常见肿瘤相关死亡原因之一,具有极高的潜在转移风险。目前众多治疗方案的效果并不尽人意,亟需探索新的治疗方法。在纳米医学领域,氧化铈纳米颗粒(cerium oxide nanoparticles,CNPs)可通过其氧空位介导的化学反应性显示出抗癌活性。然而,CNPs在胃癌中的实际效果并不明确。因此,通过可控合成球形氧化铈纳米颗粒(spherical cerium oxide nanoparticles,Ceria-SNPs)并对其表征,探讨Ceria-SNPs对人胃癌细胞MKN-45的作用。结果发现,Ceria-SNPs的平均粒径为14 nm,比表面积为59.54 m^(2)·g^(-1)。将不同浓度的Ceria-SNPs与胃癌细胞共培养,并在体外和体内检测细胞增殖和迁移能力。结果发现,高浓度(10.0μg·ml^(-1))的Ceria-SNPs能抑制胃癌细胞的增殖和迁移,且该过程是通过增加细胞内活性氧(reactive oxygen species,ROS)水平实现的;裸鼠皮下成瘤与腹腔转移模型进一步验证效能和生物安全性。Ceria-SNPs可以通过增加细胞内ROS水平抑制胃癌恶性生物学行为,而根据特殊应用需求可控合成CNPs为其进一步开展医用研究提供了材料支撑。Gastric cancer(GC)has the fifth highest incidence rate and fourth highest mortality rate worldwide,with a highly significant risk of metastasis.Due to the difficulty in detecting early symptoms of gastric cancer and the low rate of gastric endoscopy screening,most patients are already in the advanced stage at the time of diagnosis.Comprehensive treatment is the overall strategy for gastric cancer treatment,with surgery being the main focus,while chemotherapy can improve the survival rate and quality of life for patients with locally advanced unresectable or metastatic gastric cancer.The key to gastric cancer treatment is early detection,diagnosis,and appropriate treatment methods.The treatment efficacy of gastric cancer,however,is still far from satisfactory.With the 5-year survival rate for advanced gastric cancer lingering below 30%,the primary causes of cancer-related fatalities persist as tumor invasion and metastasis.Thus,a pressing necessity emerges for intensive exploration and advancement to enhance the survival rate and therapeutic consequences for patients grappling with gastric cancer,by discovering and employing increasingly efficacious drugs that can effectively stymie the proliferation and migration of gastric cancer cells.Nanotechnology,being a"disruptive technology",has demonstrated immense potential for enhancing numerous disease treatments and diagnoses.With the escalating demand for individualized and accurate drug therapies,there is an escalating interest in the advancement of nano particles.These nano particles have already played diverse therapeutic roles in various diseases including chemotherapy,photodynamic therapy,radiation therapy,and immunotherapy.The continuing advancements in nanotechnology will cater to medical requirements more effectively,offering patients more personalized treatments and more efficient drug delivery systems.Research has found that in the field of nanomedicine,cerium oxide nanoparticles(CNPs)have the potential to be a highly effective treatment method for a wide r

关 键 词：氧化铈纳米颗粒(CNPs) 胃癌(GC) 活性氧(ROS) 纳米医学 细胞增殖 

分 类 号：R318.08[医药卫生—生物医学工程]