糖苷水解酶基因FvGH16-2参与拟轮枝镰孢菌生长发育及致病性  

Glycoside hydrolase gene FvGH16-2 is involved in the growth and pathogenicity of mycotoxigenic fungus Fusarium verticillioides

作　　者：任志国 刘宁[1,2] 陈悦[2,3] 孙蔓莉 曹志艳 董金皋[1,2] Ren Zhiguo;Liu Ning;Chen Yue;Sun Manli;Cao Zhiyan;Dong Jingao(College of Plant Protection,Hebei Agricultural University,Baoding 071000,Hebei Province,China;Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071000,Hebei Province,China;College of Life Science,Hebei Agricultural University,Baoding 071000,Hebei Province,China)

机构地区：[1]河北农业大学植物保护学院,保定071000 [2]河北农业大学,河北省植物生理与分子病理学重点实验室,华北作物改良与调控国家重点实验室,保定071000 [3]河北农业大学生命科学学院,保定071000

出　　处：《植物保护学报》2024年第2期342-351,共10页Journal of Plant Protection

基　　金：国家现代农业产业技术体系(CARS-02),河北省重点研发计划(20326502D);河北省“三三三人才工程”人才培养项目(A202102005)。

摘　　要：为明确糖苷水解酶16(glycoside hydrolase,GH16)家族基因在拟轮枝镰孢菌Fusarium verticillioides生长发育和致病过程中的作用,利用生物信息学分析软件对其GH16家族成员进行鉴定,采用转录组分析及实时荧光定量PCR技术对GH16家族成员在不同培养温度下的表达水平进行检测,并通过遗传转化法对家族成员FvGH16-2基因进行敲除及回补,分析其在拟轮枝镰孢菌生长发育及致病过程中的作用。结果显示,在拟轮枝镰孢菌基因组中共鉴定到23个GH16家族基因,其中FvCH16-2、FvCH16-4和FvCH16-12等多个上调表达基因与病菌的生长和抗逆性相关。敲除FvGH16-2基因导致拟轮枝镰孢菌的生长速率及产孢能力降低,细胞壁完整性受损,对H2O2更加敏感,同时对玉米籽粒和茎秆的致病力减弱。表明FvGH16-2基因在拟轮枝镰孢菌生长发育和致病过程中发挥着重要作用,是拟轮枝镰孢菌细胞壁完整性和致病性所必需的。To clarify the role of glycoside hydrolase 16(GH16)family genes in the growth,development,and pathogenesis of mycotoxigenic fungus Fusarium verticillioides,bioinformatics software was used to identify GH16 family members,and transcriptome and real-time fluorescence quantitative PCR techniques were used to analyze the expression levels of GH16 family members at different culture temperatures.The FvGH16-2 gene was knocked out and replaced by genetic transformation to analyze its role in growth,development,and pathogenesis of F.verticillioides.The results showed that a total of 23 GH16 family genes were identified in F.verticillioides genome,among which FvCH16-2,FvCH16-4,FvCH16-12 and other up-regulated genes were associated with the growth and stress resistance of pathogens.FvGH16-2 gene knockout resulted in decreased growth rate and conidial production of F.verticillioides,impaired cell wall integrity,and increased sensitivity to H2O2.In addition,FvGH16-2 gene knockout reduced the pathogenicity of F.verticillioides on maize seeds and maize stalks.These results indicated that the FvGH16-2 gene played an important role in the growth,development,and pathogenesis of F.verticillioides,and was essential for the integrity of the cell wall and pathogenicity of F.verticillioides.

关 键 词：糖苷水解酶家族 拟轮枝镰孢菌 玉米病害 基因表达 功能分析 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]