氨基酸基防污可注射自融合水凝胶的制备及其在术后防粘连中的应用  

Injectable and self-fused hydrogels with antifouling capability based on amino acid derivatives for postoperative anti-adhesion application

作　　者：李萌 张梦园 刘正 谢瑞琳 杨宇轩 申锴翔 杨爱民 成一龙 Meng Li;Mengyuan Zhang;Zheng Liu;Ruilin Xie;Yuxuan Yang;Kaixiang Shen;Aimin Yang;Yilong Cheng(Engineering Research Center of Energy Storage Materials and Devices,Ministry of Education,School of Chemistry,Xi’an Jiaotong University,Xi’an,710049,China;Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University,Xi’an,710049,China;Department of Nuclear Medicine,the First Affiliated Hospital of China,Xi’an Jiaotong University,Xi’an,710049,China)

机构地区：[1]Engineering Research Center of Energy Storage Materials and Devices,Ministry of Education,School of Chemistry,Xi’an Jiaotong University,Xi’an,710049,China [2]Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University,Xi’an,710049,China [3]Department of Nuclear Medicine,the First Affiliated Hospital of China,Xi’an Jiaotong University,Xi’an,710049,China

出　　处：《Science China Materials》2024年第5期1521-1532,共12页中国科学（材料科学）（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(52322309 and 52173139);the"Young Talent Support Plan"of Xi’an Jiaotong University;the Fundamental Research Funds for the Central Universities(xzy022022015 and xzy022023018)。

摘　　要：设计和制备具有优异防污能力的可注射防粘连材料对预防手术后组织和器官的粘连具有重要的意义.在此,我们将含有羟基的氨基酸衍生物引入水凝胶的制备中,通过N-丙烯酰丝氨酸(ASer)的自由基聚合制备了一种新型的氢键交联水凝胶(PASer).结果发现一个甲基取代基的存在会对分子间氢键相互作用产生明显的影响:与聚(N-丙烯酰苏氨酸)(PAThr)水凝胶相比,PASer水凝胶具有较低的成胶浓度和增强的力学性能.值得一提的是,亲水性羟基使PASer水凝胶能够有效抵抗蛋白质吸附和细胞粘附.PASer水凝胶具有优异的力学性能、可注射性、自融合性、良好的生物相容性和适宜的体内停留时间,符合临床对术后抗粘连材料的要求.体内研究实验结果表明,PASer水凝胶在建立的小鼠腹壁缺损模型中可以有效预防术后腹膜粘连发生.The design and fabrication of injectable barriers with promising antifouling capability to prevent the formation of adhesions after surgery remains challenging.Herein,we introduce amino acid derivative with hydroxyl group(N-acryloyl serine(ASer))into the preparation of hydrogels to develop a novel hydrogen bond-crosslinked hydrogel(PASer)by free radical polymerization.It is found that one substituted methyl group can cause considerable disruption to the hydrogen bonding interaction and result in increased initial gelling concentration and significant decrease in the mechanical properties of poly(N-acryloyl threonine)(PAThr)hydrogel compared with PASer hydrogel.Moreover,it is worth noting that the hydrophilic hydroxyl groups enable PASer hydrogel to efficiently resist protein adsorption and cell adhesion.The PASer hydrogel possesses excellent mechanical performance,injectability,self-fusion,good biocompatibility and appropriate in vivo residence time,which are consistent with the requirements of postoperative anti-adhesion materials in clinical practice.In vivo studies reveal that the advanced hydrogel shows satisfactory preventive effect on postoperative peritoneal adhesions in the established mice abdominal wall defect model.

关 键 词：HYDROGEL INJECTABILITY ANTIFOULING amino acid abdominal adhesion 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]