Dammarane-type triterpenoids from Gynostemma longipes and their protective activities on hypoxia-induced injury in PC12 cells  

作　　者：LIANG Haizhen CHEN Xiaojuan LI Qi ZHANG Mengmeng LU Pengxin ZHANG Jie SONG Juan ZHANG Tao GUO Baolin MA Baiping 

机构地区：[1]Department of Pharmaceutical Sciences,Beijing Institute of Radiation Medicine,Beijing 100850,China [2]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China [3]AnKang Chia Tai Pharmaceutical Co.,Ltd.,Shaanxi 725000,China

出　　处：《Chinese Journal of Natural Medicines》2024年第5期466-480,共15页中国天然药物（英文版）

摘　　要：Sixteen new dammarane-type triterpenoid saponins(1-16)featuring diverse structural variations in the side chain at C-17,along with twenty-one known analogues(17-37),have been isolated from the rhizomes of Gynostemma longipes C.Y.Wu,a plant renowned for its medicinal and edible properties.The structural elucidation of these compounds was accomplished through comprehensive analyses of 1D and 2D NMR and HRMS spectroscopic data,supplemented by comparison with previously reported data.Subsequent assays on the isolates for their protective effects against hypoxia-induced damage in pheochromocytoma cells(PC12 cells)revealed that nine saponins exhibited significant anti-hypoxic activities.Further investigation into the anti-hypoxia mechanisms of the representative saponins demonstrated that compounds 22 and 36 markedly reduced the levels of hypoxia-induced apoptosis.Additionally,these compounds were found to decrease the release of lactate dehydrogenase(LDH)and malondialdehyde(MDA),while increasing the activity of superoxide dismutase(SOD),thereby indicating that the saponins could mitigate hypoxia-induced injuries by ameliorating apoptosis and oxidative stress.These findings offer substantial evidence for the future utilization and development of G.longipes,identifying dammarane-type triterpenoid saponins as its active anti-hypoxic constituents.

关 键 词：Gynostemma longipes Dammarane-type saponins Anti-hypoxia effect Apoptosis 

分 类 号：R284.1[医药卫生—中药学]