土家族药材冷水七指纹图谱及根皮苷含量测定的研究  

Fingerprint and Phlorizin Content Assay of Tujia Ethnomedicinal Herb Impatientis Pritzelii Rhizoma

作　　者：肖凌 刘梦涵 徐梦麟 胡敏 汤俊 XIAO Ling;LIU Menghan;XU Menglin;HU Min;TANG Jun(NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei,Engineering Research Center for Drug Quality Control,Hubei Institute for Drug Control,Wuhan 430075,China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery(Wuhan University),Ministry of Education,School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,China)

机构地区：[1]湖北省药品监督检验研究院,国家药品监督管理局中药质量控制重点实验室,湖北省药品质量检测与控制工程技术研究中心,武汉430075 [2]武汉大学药学院,组合生物合成与新药发现教育部重点实验室,武汉430071

出　　处：《中国药学杂志》2024年第9期827-833,共7页Chinese Pharmaceutical Journal

基　　金：湖北省重点研发计划项目“湖北中药质控标准物质研制及技术平台建立”资助(2020ACA007);国家药品监督管理局注册司民族药专项“特色民族药检验方法的示范性研究”资助(NIFDC-TCM2021-049-MZY011)。

摘　　要：目的提高土家族药材冷水七的质量控制方法,建立该药材指纹图谱及含量测定方法。方法指纹图谱采用超高效液相色谱法(UPLC),ACQUITY UPLC BEH C18柱,根皮苷含量测定采用高效液相色谱法(HPLC),Eclipse XDB-C18柱,流动相均为乙腈-0.1%甲酸梯度洗脱,检测波长为283 nm,对冷水七及其易混品(同属块节凤仙花、滇水金凤、黄金凤、水金凤的根茎)进行比较研究。结果指纹图谱中9个特征峰可作为冷水七的特征峰,其中4个峰分别与儿茶素、表儿茶素、东莨菪素及根皮苷相对应;该指纹图谱反映了冷水七化学成分的分布,且可与其易混品区分。根皮苷为首次在冷水七中发现,16批冷水七中根皮苷含量范围为0.007%~0.085%,易混品未检出根皮苷。结论本研究建立的冷水七指纹图谱及其根皮苷含量测定方法专属、准确、有效,可用于冷水七药材的质量控制。OBJECTIVE To establish the fingerprint and content determination method of Impatientis Pritzelii Rhizoma to improve the quality control method of Tujia ethnomedicinal herb Impatientis Pritzelii Rhizoma.METHODS The fingerprint was established by UPLC with ACQUITY UPLC BEH C18 column.Phlorizin content assay was performed by HPLC with Eclipse XDB-C18 column.The mobile phases for both methods were composed of acetonitrile and 0.1%formic acid with different gradient elution procedures.The detection wavelength was set at 283 nm.The comparative study was carried out between Impatientis Pritzelii Rhizoma and its adulterants(such as the roots and rhizomes of Impatiens pinfanensis,I.uliginosa,I.siculifer and I.noli-tangere).RESULTS Nine main chromatographic peaks in the fingerprint could be served as the characteristic peaks of Impatientis Pritzelii Rhizoma,four of which were corresponding to catechin,epicatechin,scopolamine and phlorizin,respectively.The established fingerprint could reflect the distribution of the chemical components of Impatientis Pritzelii Rhizoma,which was distinguished from four adulterants.Phlorizin was found and determined in Impatientis Pritzelii Rhizoma for the first time.The contents in 16 batches of Impatientis Pritzelii Rhizoma ranged from 0.007%to 0.085%,while no phloridin was found in the adulterants.CONCLUSION The established methods for the fingerprint and phlorizin content determination of Impatientis Pritzelii Rhizoma are specific,accurate and effective,which could be used for its quality control.

关 键 词：冷水七 凤仙花属 根皮苷 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]