具有抗蛋白黏附与抗菌性能的蝌蚪状单链粒子刷的构筑  

Rational Design of Tadpole-like Single-chain Particle Brush with Anti-protein and Antibacterial Performance

作　　者：朱杰 吴乃冰 赵晓雅 范艳斌 黄霞芸 陈道勇[1] Jie Zhu;Nai-bing Wu;Xiao-ya Zhao;Yan-bin Fan;Xia-yun Huang;Dao-yong Chen(The State Key Laboratory of Molecular Engineering of Polymers,Department of Macromolecular Science,Fudan University,Shanghai 200438;The Dow Chemical Company,Shanghai 201203)

机构地区：[1]聚合物分子工程国家重点实验室复旦大学高分子科学系,上海200438 [2]陶氏化学公司,上海201203

出　　处：《高分子学报》2024年第7期900-909,共10页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号22071032,51721002,52293473);科技部国家重点研发计划(项目号2022YFA1203001);陶氏化学公司资助项目。

摘　　要：基于蝌蚪状单链粒子与硅片表面的多位点协同非共价作用,发展了一种兼具操作便捷性和优异“生物防污”性能的PEG刷接枝策略.通过原子转移自由基聚合(ATRP)制备了聚乙二醇-b-聚(4-乙烯基吡啶)(PEG-b-P4VP)嵌段共聚物,利用“静电介导”法对PEG-b-P4VP的P4VP嵌段进行链内交联,进一步加入过量的碘乙烷,制备得到P4VP“头部”完全季铵化的蝌蚪状单链粒子(TSCP-Q).在简单的溶液浸泡接枝过程中,充分交联塌缩的“头部”与硅片表面通过多位点非共价协同作用,显著减少其在表面的吸附面积,而充分舒展的PEG“尾部”则通过体积排斥效应,确保了TSCP-Q刷在表面的接枝均匀性.因此,所获得的TSCP-Q刷可在硅片表面实现了稳定且均匀地接枝,其中PEG“尾部”位于P4VP“头部”锚定层上方并有效遮蔽锚定层电荷.实验表明,TSCP-Q刷具有优异的抗牛血清蛋白(BSA)黏附性能(4.8 ng/cm2),并且能够有效抑制金黄色葡萄球菌(S.aureus)的繁殖(抗菌率可达81%).Based on the synergistic non-covalent interaction of tadpole-like single-chain particles with multiple sites on the wafer surface,a PEG brush grafting strategy has been developed that combines ease of handling and excellent“biofouling”properties.Specifically,the block copolymer poly(ethylene glycol)-b-poly(4-vinylpyridine)(PEG-b-P4VP)was synthesized by atom transfer radical polymerization(ATRP).The P4VP block was then intrachain cross-linked using the“electrostatic-mediated”strategy,and the P4VP“head”was completely quaternized by further addition of excess ethyl iodide,yielding tadpole-like single-chain particles with completely quaternized“heads”(TSCP-Q).By simple solution immersion grafting method,the completely cross-linked and collapsed“head”significantly reduces its adsorption area on the silicon wafer through multi-site non-covalent synergistic interaction,while the extended PEG“tail”ensures grafting uniformity of TSCP-Q brushes on the surface through volume repulsion effect.As a result,the obtained TSCP-Q brushes achieve stable and uniform grafting on the silicon wafer,with PEG“tail”overlying the P4VP“head”anchoring layer and effectively masking the charge of the anchoring layer.The TSCP-Q brushes were shown to have excellent anti-bovine serum albumin(BSA)adsorption(4.8 ng/cm2)and could effectively inhibit the colonization of Staphylococcus aureus(S.aureus)(killing efficacy up to 81%).

关 键 词：蝌蚪状单链粒子 聚合物刷 聚乙二醇 抗蛋白黏附 抗菌 

分 类 号：TQ317[化学工程—高聚物工业]