针刺捻转补泻手法对自发性高血压大鼠神经递质及炎症因子的影响  

Effects of Acupuncture with Twirling Reinforcing-Reducing Manipulation on Neurotransmitters and Inflammatory Factors in Spontaneously Hypertensive Rats

作　　者：张静[1] 刘继鹏 岳炳南 高辛瑜 张琳[1] 刘清国[1] Zhang Jing;Liu Jipeng;Yue Bingnan;Gao Xinyu;Zhang Lin;Liu Qingguo(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中医急症》2024年第6期959-962,981,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82074553)。

摘　　要：目的通过观察针刺捻转补泻手法对自发性高血压大鼠(SHR)下丘脑室旁核(PVN)中神经递质γ-氨基丁酸(GABA)、谷氨酸(Glu)含量以及血清中炎症因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平表达的影响,探寻针刺调控血压的作用机制。方法27只雄性SHR大鼠随机分配到补法组、泻法组和模型组中。9只Wistar-Kyoto(WKY)大鼠作为空白组。空白组和模型组只进行捉抓固定不做针刺干预,补法组和泻法组均取双侧的太冲穴进行针刺干预。各组每日上午干预1次,周期为14 d,2周之间休息1 d。于针刺第0、3、7、14日定期测量大鼠尾动脉收缩压并记录。实验第14日取材,采用ELISA法检测大鼠PVN中GABA、Glu的含量,以及检测血清中炎症因子IL-6、TNF-α的含量,最后使用HE染色法观察PVN组织病理学改变。结果与模型组相比,补法组和泻法组均能显著降低SHR大鼠血压(P<0.01),且泻法组降压效果更为显著(P<0.05)。针刺后,与模型组相比,补法组和泻法组PVN组织中GABA含量显著增加,Glu含量显著降低(均P<0.01);补法组和泻法组血清中IL-6、TNF-α水平相比模型组显著下降(P<0.05或P<0.01)。HE染色结果显示:与模型组相比,补法组和泻法组可改善病变情况,细胞总数增多。结论针刺捻转补泻手法可明显降低SHR大鼠血压,且捻转泻法降压效果更为显著,其作用机制可能与PVN中神经递质GABA含量升高、Glu含量降低,以及炎症因子IL-6、TNF-α水平下降相关。Objective:To investigate the mechanism of blood pressure regulation by observing the effects of acupuncture with twirling reinforcing-reducing manipulation on the contents of neurotransmitters-aminobutyric acid(GABA)and glutamate(Glu)in the hypothalamic paraventricular nucleus(PVN),as well as the expression of inflammatory factors interleukin-6(IL-6)and tumour necrosis factor-α(TNF-α)levels in serum of spontaneously hypertensive rats(SHR).Methods:A total of 27 male SHR were randomly assigned to twirling-reinforcing manipulation group,twirling-reducing manipulation group and model group,and another 9 Wistar-Kyoto(WKY)rats were used as blank group.The blank group and model group were only immobilized by grasping without acupuncture intervention,while acupuncture intervention was performed bilaterally on the"Taichong acupoint"in both the twirling-reinforcing manipulation group and twirling-reducing manipulation group.The groups were treated once a day in the morning for a period of 14 days,with 1 day of rest between the 2 weeks.The systolic blood pressure of the rat tail artery was measured and recorded periodically on days 0,3,7,and 14 of acupuncture.Samples were collected after the 14th day of the experiment,and the contents of GABA and Glu in the PVN tissues of rats were determined by ELISA,as well as the levels of inflammatory factors IL-6 and TNF-αin serum.Finally,histopathologic changes in the PVN tissues were observed by HE staining.Results:Compared with the model group,the twirling-reinforcing manipulation group and twirling-reducing manipulation group could significantly reduce the blood pressure of SHR rats(P<0.01),and the antihypertensive effect of the twirling-reducing manipulation group was more significant(P<0.05).After acupuncture,GABA contents were significantly increased and Glu contents were significantly decreased in the PVN tissues of the twirling-reinforcing manipulation group and twirling-reduc ing manipulation group compared with the model group(all P<0.01).The serum levels of IL-6 and TNF-

关 键 词：高血压 针刺 捻转补泻手法 下丘脑室旁核 神经递质 炎症因子 自发性高血压大鼠 

分 类 号：R544.1[医药卫生—心血管疾病]