三子利水颗粒对心衰肺水肿大鼠肺组织液体转运相关蛋白表达的影响  

Effects of Sanzi Lishui Granules on the Expression of Fluid Transport-Related Proteins in Lung Tissue of Rats with Heart Failure and Pulmonary Edema Based on the Theory of"Lung Regulates Water Channels"

作　　者：张晓华[1] 赵宏宇[1] 宋智冰[1] 刘淑荣[1] 钱锋[1] 王玉[1] 于清华[1] 李雪梅 王邵鹏 杜星辰 Zhang Xiaohua;Zhao Hongyu;Song Zhibing;Liu Shurong;Qian Feng;Wang Yu;Yu Qinghua;Li Xuemei;Wang Shaopeng;Du Xingchen(The First Clinical Hospital of Jilin Academy of Traditional Chinese Medicine and Pharmacology,Jilin,Changchun 130031,China)

机构地区：[1]吉林省中医药科学院第一临床医院,吉林长春130031 [2]长春中医药大学,吉林长春130021

出　　处：《中国中医急症》2024年第6期968-972,976,共6页Journal of Emergency in Traditional Chinese Medicine

基　　金：吉林省科技厅自然基金项目(20210101225JC)。

摘　　要：目的通过观察三子利水颗粒对心衰肺水肿大鼠肺组织病理组织学、AQP1、AQP5、ENaC蛋白及mRNA表达的影响探讨其治疗心衰肺水肿的机制。方法选择雄性SD大鼠,通过结扎腹主动脉、腹腔注射氯化铵造成心衰急性肺水肿模型,随机分为模型组、西药组、中药低剂量组、中药高剂量组、中药低剂量+西药组、中药高剂量+西药组。给药1周后观察各组大鼠肺组织含水率、病理组织学及AQP1、AQP5、ENaC蛋白及mRNA表达水平。结果与模型组比较,各给药组肺含水量均减少(P<0.05);组织细胞间的炎性浸润和肺泡间的充血症状均改善。AQP1、AQP5蛋白表达均不同程度地升高,中药低剂量组AQP1差异有统计学意义(P<0.05),AQP5差异无统计学意义(P>0.05),其余给药组差异有统计学意义(P<0.01)。ENaC蛋白表达均不同程度地升高,空白对照组、中药低剂量组+西药组、中药高剂量组+西药组差异有统计学意义(P<0.05),其余各组差异无统计学意义(P>0.05)。AQP1、AQP5mRNA表达均不同程度地升高,中药低剂量组+西药组、中药高剂量组+西药组差异有统计学意义(P<0.01)。ENaC mRNA表达均不同程度地升高,中药低剂量组+西药组差异有统计学意义(P<0.05),西药组、中药高剂量组+西药组差异有统计学意义(P<0.01)。中药低、高剂量组差异无统计学意义(P>0.05)。结论三子利水颗粒可通过升高AQP1、AQP5、ENaC蛋白表达及mRNA表达含量来改善上皮细胞病理组织形态,达到清上焦肺水,缓解心衰肺水肿体征的目的。Objective:To explore the mechanism of Sanzi Lishui Granules in the treatment of heart failure and pulmonary edema by observing its effects on the pathological histology,AQP1,AQP5,ENaC protein and mRNA expression in lung tissue of rats with heart failure and pulmonary edema.Methods:Male SD rats were selected and randomly divided into the model group,western medicine control group,Chinese medicine low dose group,Chinese medicine high dose group,Chinese medicine low dose+western medicine group,Chinese medicine high dose+western medicine group by ligating the abdominal aorta and injecting ammonium chloride intraperitoneally to create a model of heart failure and acute pulmonary edema.Changes in water content,pathological histology and expression of AQP1,AQP5,ENaC protein and mRNA in lung tissue of rats in each group were observed after 1 week of administration.Results:Compared with the model group,the lung water content of each treatment group decreased(P<0.05);The inflammatory infiltration between tissue cells and congestion symptoms between alveoli have both improved.The expression of AQP1 and AQP5 proteins increased to varying degrees.The low-dose group of traditional Chinese medicine showed statistically significant differences in AQP1(P<0.05),while there was no statistically significant difference in AQP5(P>0.05).The other treatment groups showed statistically significant differences(P<0.01).The expression of ENaC protein increased to varying degrees,with significant differences observed in the blank control group,low-dose Chinese medicine group+western medicine group,and high-dose Chinese medicine group+western medicine group(P<0.05),while no significant differences were observed in the other groups(P>0.05).The expression of AQP1 and AQP5mRNA increased to varying degrees,and there was a statistically significant difference between the low-dose group of traditional Chinese medicine and the western medicine group,as well as the high-dose group of traditional Chinese medicine and the western medicine group(P<0.01)

关 键 词：肺水肿 三子利水颗粒 水通道蛋白1 水通道蛋白5 上皮细胞钠离子通道 大鼠 

分 类 号：R285.5[医药卫生—中药学]