大柴胡汤通过TLR4/NF-κB通路改善重症中暑大鼠炎症的机制研究  

Study on the Mechanism of Dachaihu Decoction in Improving Inflammation in Rats with Severe Heat Stroke Through the TLR 4/NF-κB Pathway

作　　者：陈冬杰 黄永莲 彭晓洪 黄亚秀 黄发根 张爱婷 王评 Chen Dongjie;Huang Yonglian;Peng Xiaohong;Huang Yaxiu;Huang Fagen;Zhang Aiting;Wang Ping(Shenzhen Hospital of Bejing University of Traditional Chinese Medicine(Longgang),Guangdong,Shenzhen 518000,China)

机构地区：[1]北京中医药大学深圳医院(龙岗),广东深圳518000

出　　处：《中国中医急症》2024年第6期973-976,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：广东省深圳市龙岗区医药卫生科技计划项目(LGKCYLWS2020072);广东省深圳市龙岗区科技创新专项基金医疗卫生科技计划项目(LGWJ2022-68)。

摘　　要：目的观察大柴胡汤对重症中暑大鼠炎症的治疗效果并探寻其作用机制。方法SPF级SD雄性大鼠60只随机分为空白对照组、模型组、中药低剂量组、中药高剂量组。将大鼠置于高温气候动物培养箱内[温度(40.50±0.50)℃,湿度(65.00±2.00)%]制备经典中暑模型,模型制备成功后6 h开始灌胃给药,8 h 1次,连续2 d。在造模成功后6、24、48 h各处死5只大鼠,检测血清白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α),同时取回肠组织,采用蛋白质免疫印迹试验测定肠道组织中TLR4、核转录因子-κB(NF-κB)蛋白表达。结果与空白对照组比较,模型组、中药低剂量组和中药高剂量组各时间点血清IL-1、TNF-α水平和肠道组织中TLR4、NF-κB蛋白表达水平均升高(P<0.05或P<0.01);中药低、高剂量组造模后24、48 h血清TNF-α、IL-1水平和肠道组织中TLR4、NF-κB的蛋白表达水平均较模型组显著下降(P<0.05)。中药高剂量组TNF-α、TLR4、NF-κB蛋白表达水平在造模后24、48 h较低剂量组明显下降,而IL-1水平在造模后48 h明显下降(P<0.05)。结论大柴胡汤能抑制TLR4/NF-κB信号通路,减轻肠道炎症反应,从而起到保护重症中暑大鼠的作用。Objective:To investigate the effect of Dachaihu Decoction in improving inflammation in rats with severe heat stroke and to investigate the mechanism of action.Methods:A total of 60 male rats were randomly divided into the blank control group,model group,standard dose traditional chinese medicine group and high dose traditional chinese medicine group.The classic heat stroke model was prepared by placing rats in a high temperature climate animal incubator[temperature(40.50±0.50)℃,humidity(65.00±2.00)%].After successful preparation,the model was administered by gavage q8h at 6 h for 2 consecutive days.5 rats were killed at 6 h,24 h and 48 h after successful modeling,and interleukin-1(IL-1),tumor necrosis factor-α(TNF-α),and ileum tissue were collected.The protein expression of TLR4 and NF-κB in intestinal tissues was determined by protein immunoblotting test.Results:Compared with the blank control group,the serum levels of IL-1 and TNF-αand the expression levels of TLR4 and NF-κB proteins in intestinal tissues were increased in the model group,the low-dose group and the high-dose traditional chinese medicine group(P<0.05 or P<0.01).The serum levels of TNF-αand IL-1 and the protein expression levels of TLR4 and NF-κB in intestinal tissues were significantly decreased at 24 and 48 h after the model group(P<0.05).The protein expression levels of TNF-α,TLR4 and NF-κB in the high-dose traditional chinese medicine group decreased significantly at 24 and 48 h after modeling,while the levels of IL-1 decreased significantly at 48 h after modeling(P<0.05).Conclusion:Dachaihu Decoction can inhibit TLR 4/NF-κB signaling pathway,reduce intestinal inflammation and gastrointestinal damage,and play a role in protecting the rats with severe heat stroke.

关 键 词：重症中暑 大柴胡汤 TLR4/NF-κB通路 炎症反应 大鼠 

分 类 号：R285.5[医药卫生—中药学]