湖南多花黄精快速繁殖体系的建立  

A Rapid Propagation System for Polygonatum cyrtonema in Hunan

作　　者：唐铭鑫 符天昊 杨韬坛 沈子豪 龚海健 欧跃红 刘叶蔓[1] 宁露云 TANG Ming-xin;FU Tian-hao;YANG Tao-tan;SHEN Zi-hao;GONG Hai-jian;OU Yue-hong;LIU Ye-man;NING Lu-yun(Hunan University of Chinese Medicine,Changsha 410208,PRC;Loudi Zhifang Biotechnology Co.,Ltd.,Loudi 417000,PRC)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]娄底市知方生物科技有限公司,湖南娄底417000

出　　处：《湖南农业科学》2024年第5期7-12,共6页Hunan Agricultural Sciences

基　　金：湖南省自然科学基金项目(2023JJ40482);湖南中医药大学2022年度校级大学生创新训练项目;湖南省教育厅科学研究项目(22C0189);2023年湖南省重点研发计划(2023SK2046);2022年度中医药民族医药国际联合实验室开放基金(2022GJSYS09);湖南中医药大学校级科研项目(2021XJJJ015)。

摘　　要：该研究分别以叶片、根状茎、芽以及种子为外植体,基于前人研究对培养基配方进行优化,共确定了21种培养基配方,从中筛选适用于湖南多花黄精的最佳萌发培养基、最佳增殖培养基以及最佳生根培养基配方,建立了湖南多花黄精快速繁殖体系。结果表明:以当年采收的种子为外植体,消毒后纵切再接种培养效果较好;最佳萌发培养基配方为:B5+1.0 mg/L 6-BA+0.5 mg/L KT+30 g/L蔗糖+6.0 g/L琼脂+2.0 mg/L GA_(3);最佳增殖培养基配方为:MS+4.0 mg/L 6-BA+0.2 mg/L NAA+30 g/L蔗糖+6.5 g/L琼脂;最佳生根培养基配方为:1/2 MS+1.0 mg/L NAA+30 g/L蔗糖+6.5 g/L琼脂。该研究为湖南多花黄精组培苗的生产奠定了坚实的理论基础,为进一步扩大多花黄精的人工种植规模提供了技术支撑。This study aimed to establish a rapid propagation system for Polygonatum cyrtonema in Hunan.With the leaves,rhizomes,buds,and seeds as explants,21 media were designed based on the available studies,and the optimal media for germination,propagation,and rooting of P.cyrtonema were selected.The results indicated the fresh seeds harvested in the current year were the best explants,and those disinfected and cut longitudinally showcased good performance in tissue culture.The optimal medium for germination was B5+1.0 mg/L 6-BA+0.5 mg/L KT+30 g/L sucrose+6.0 g/L agar+2.0 mg/L GA_(3).The optimal medium for propagation was MS+4.0 mg/L 6-BA+0.2 mg/L NAA+30 g/L sucrose+6.5 g/L agar.The optimal medium for rooting was 1/2 MS+1.0 mg/L NAA+30 g/L sucrose+6.5 g/L agar.The findings lay a theoretical foundation for producing tissue culture seedlings and provide technical support for expanding the planting scale of P.cyrtonema in Hunan.

关 键 词：多花黄精 组织培养 萌发 增殖 生根 

分 类 号：S567.239[农业科学—中草药栽培]