基于近红外漫射光谱的离体血氧测量技术研究  

In Vitro Measurement of Blood Oxygenation Based on Near⁃Infrared Diffuse Optical Spectroscopy

作　　者：刘培俊 李为龙 张睿芝 高国雅 桂志国[1] 尚禹[1] LIU Peijun;LI Weilong;ZHANG Ruizhi;GAO Guoya;GUI Zhiguo;SHANG Yu(School of Information and Communication Engineering,North University of China,Taiyuan 030051,China)

机构地区：[1]中北大学信息与通信工程学院,山西太原030051

出　　处：《中北大学学报（自然科学版）》2024年第3期348-356,共9页Journal of North University of China(Natural Science Edition)

基　　金：山西省基础研究计划项目(202203021221097,202203021211100);中北大学研究生科技立项(2022180502,20221831,20221833);山西省研究生教育创新项目(2022Y621)。

摘　　要：基于近红外漫射光谱技术(Near-Infrared Diffuse Optical Spectroscopy,NIRS)和修正的比尔朗伯定律(Modified Beer-LambertLaw,MBLL)提出了一种适用于离体血液的新型氧含量测量方法,同时开发了血氧实时监测系统以实现体外循环血氧参数的连续监测。NIRS利用脱氧血红蛋白和氧合血红蛋白对两种波长近红外光吸收率的差异来计算组织的吸收和散射系数,朗伯-比尔定律是表征光强衰减与光子传输路径关系的数学表达式,将二者结合可估算血红蛋白的吸收系数;与传统的测量方式不同,本文所提出的透射式测量方法,通过双波长(660~904 nm)光源探头交替切换可获取多参数的光学信号,信号实时上传至采集界面(LabVIEW),经过对数化处理,并结合创新的血氧算法得到血液吸收系数、血氧饱和度与光信号的线性模型。为了验证本文研究方案的可行性,开展了液态仿体实验和离体血溶液实验,以滴入溶液墨水体积(仿体实验)和酵母溶液体积(血溶液实验)为变量,通过调控溶液浓度改变光密度变化幅度,证实二者均与光密度(DO)具有良好的线性关系(r^(2)>0.95,p<0.01)。本研究所提出的新型血氧测量方法和血氧实时监测系统适合于离体血液的血氧测量,数据准确可靠、重复稳定性好。Based on near-infrared spectroscopy(NIRS)and modified Beer-Lambert law(MBLL),a new method for measuring oxygen content in vitro blood was proposed,at the same time,a blood oxygen satu-ration real-time monitoring system was developed to realize continuous monitoring of blood oxygen param-eters during cardiopulmonary bypass.NIRS computes the absorption and scattering coefficients of tissues by using the difference in the absorption rates of the two wavelengths of near-infrared light by deoxyhemo-globin and oxygenated hemoglobin.Beer-Lambert law is a mathematical expression that characterizes the relationship between light intensity attenuation and photon transmission path.Combining the two can esti-mate the absorption coefficient of hemoglobin.Different from the traditional measurement method,the transmission measurement method proposed in this paper can obtain multi-parameter optical signal by alter-nating the probe of double wavelength(660–904 nm)light source.The signal was uploaded to the acquisi-tion interface(LabVIEW)in real time and processed by logarithm,a linear model of blood absorption coefficient,oxygenation and optical signals was developed using an innovative blood oxygen algorithm.In order to verify the feasibility of the research scheme,the experiments of liquid phantom and in vitro blood solution were carried out,and the volume of Ink Drop(phantom experiment)and yeast(blood solution experiment)were taken as variables,by adjusting the concentration of solution to change the change ampli-tude of optical density,it was proved that both of them had a good linear relationship with optical density(DO)(r^(2)>0.95,p<0.01).The novel oximetry method and real-time oximetry monitoring system pro-posed in this study are suitable for oximetry of isolated blood,with accurate and reliable data,good repeat-ability and stability.

关 键 词：近红外漫射光谱技术 修正的比尔朗伯定律 体外循环 血氧饱和度 

分 类 号：R446.11[医药卫生—诊断学]