辣椒雄性不育分子研究进展  

Molecular Advancements of Male Sterility in Pepper

作　　者：徐晓美[1] 衡周 王恒明[1] 孙启迪 徐小万[1] XU Xiaomei;HENG Zhou;WANG Hengming;SUN Qidi;XU Xiaowan(Vegetable Research Institute,Guangdong Academy of Agriculture Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables,Guangzhou 510640;Maoming Maoshu Seed Technology Co.,Ltd.,Maoming,Guangdong 525000)

机构地区：[1]广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广州510640 [2]茂名市茂蔬种业科技有限公司,广东茂名525000

出　　处：《中国农学通报》2024年第13期27-35,共9页Chinese Agricultural Science Bulletin

基　　金：广东省基础与应用基础研究基金项目“辣椒根腐疫病候选抗性基因功能验证及其抗病分子机制研究”(2021A1515012132);广东省基础与应用基础研究基金(粤桂联合基金)项目“辣椒根腐疫病候选抗性基因CaRLK功能验证及其与Avr蛋白互作的分子机制研究”(2020A1515410005);2022年省级乡村振兴战略专项资金种业振兴项目“瓜果类蔬菜核心种质指纹图谱构建”(2022-NJS-00-005);广州市科技计划项目“多技术融合选育华南特色辣椒新品种”(2023B03J1082);广东省农业厅种业振兴与科技兴农项目“高产优质辣椒新品种选育研究”(2022-NPY-00-024)。

摘　　要：为对辣椒雄性不育分子研究取得的进展有更直观、系统、深入的了解,梳理已鉴定的辣椒核雄性不育(NMS)基因及部分NMS基因分子标记开发,重点阐述近年来核雄性不育基因精细定位及候选基因探究工作;同时回顾辣椒胞质雄性不育(CMS)相关的线粒体不育基因及其关联分子标记和CMS恢复基因分子标记开发,重点概述近年来恢复基因精细定位研究取得的进展。研究指出,精细定位NMS基因,明确候选基因并开发功能标记是辣椒NMS研究的重点,既能为探明辣椒NMS机理奠定基础又可为育种提供高效利用的分子标记。而CMS相较于NMS更为复杂,表现在遗传机制多样且更易受环境影响等方面,因此,从对CMS育性恢复基因的精细定位到基因克隆以及功能研究并探明CMS分子机制还有很长的一段路要走。In order to have a more intuitive,systematic and in-depth understanding of the progress made in molecular research on male sterility in pepper,we sorted out the identified nuclear male sterility(NMS)genes in pepper and the development of molecular markers for some of the NMS genes,and highlighted the fine mapping of the NMS genes and the exploration of the candidate genes in recent years.Meanwhile,we reviewed the mitochondrial sterile genes related to cytoplasmic male sterility(CMS)and their associated molecular markers,as well as molecular markers developed for CMS fertility restoration genes,with a focus on the progress made in recent years in fine mapping of fertility restoration genes.Finally,it was pointed out that the fine mapping of NMS genes,identification of candidate genes and development of functional markers were the main focuses of NMS research in pepper,which could not only lay the foundation for the exploration of the mechanism of NMS in pepper,but also provided molecular markers for efficient use in breeding.Compared with NMS,CMS is more complex,characterized by diverse genetic mechanisms and more susceptible to environmental influences. Therefore, there is still a long way to go from the fine mapping of CMS restorer-offertility genes to the cloning of genes and the functional studies to understand the molecular mechanisms of CMS.

关 键 词：辣椒 核雄性不育 胞质雄性不育 恢复基因 分子标记 

分 类 号：S641.3[农业科学—蔬菜学]