猕猴桃黑头病生防菌的筛选、鉴定及防效评价  

Screening,Identification and Control Effect of Biocontrol Bacteria Against Kiwifruit Black Spot Disease

作　　者：王家哲 张锋 李英梅 周搏航 付博 WANG Jiazhe;ZHANG Feng;LI Yingmei;ZHOU Bohang;FU Bo(Bio-Agriculture Institute of Shaanxi,Xi’an 710043)

机构地区：[1]陕西省生物农业研究所,西安710043

出　　处：《中国农学通报》2024年第13期109-112,共4页Chinese Agricultural Science Bulletin

基　　金：西安市科技局科技计划项目“猕猴桃黑斑病发病规律及综合防控技术研究”(21NYYF0026);西安市科技局农业重点产业链关键技术攻关项目“猕猴桃土传病害防控和土壤修复关键技术研究与示范”(22NYGG0007)。

摘　　要：研究旨在筛选对猕猴桃黑头病病原真菌(Diaporthe eres)有拮抗作用的菌株。通过平板对峙法从陕西周至县猕猴桃根际土壤中分离筛选到1株具有明显拮抗作用的菌株BKH。显微及菌落形态观察、生理生化试验和16S rDNA序列分析结果表明,该菌株为双向伯克霍尔德氏菌(Burkholderia ambifaria)。菌株BKH在平板上能显著抑制Diaporthe eres生长,抑菌率为72.5%,且对猕猴桃黑头病田间防效可达91.7%,在猕猴桃黑头病生物防治方面具有应用潜力。In order to screen the antagonistic bacteria against Diaporthe eres,strain BKH with good antagonistic effect was isolated from the rhizosphere soil of Actinidia chinensis in Zhouzhi County,Shaanxi Province by plate confrontation method.BKH strain was identified as Burkholderia ambifaria,through the methods of colony and microscopic morphology observation,physiological and biochemical test and 16S rDNA gene sequences analysis.BKH strain could significantly inhibit the growth of Diaporthe eres on the plate,and the inhibition rate was 72.5%.The field control effect of BKH strain against kiwifruit black spot disease was 91.7%,which had great potential in biological control of kiwifruit black spot disease.

关 键 词：猕猴桃黑头病 双向伯克霍尔德氏菌 生防菌 鉴定 防效评价 

分 类 号：S436.634.1[农业科学—农业昆虫与害虫防治]