一株草莓根腐病病原菌的鉴定及生物学特性分析  

Identification and Biological Characteristics Analysis of a Pathogen of Strawberry Root Rot

作　　者：王梦园 杜延全 闫加力 汪丹 张阳阳 朱建强[4] WANG Mengyuan;DU Yanquan;YAN Jiali;WANG Dan;ZHANG Yangyang;ZHU Jianqiang(Hubei Institute of Geosciences,Wuhan 430034;Hubei Provincial Test Center for Selenium Eco-environmental Effect,Wuhan 430034;Linyi Agricultural Research and Development Center,Sinochem Chemical Fertilizer Co.,LTD.,Linyi,Shandong 276000;College of Agriculture,Yangtze University,Jingzhou,Hubei 434025)

机构地区：[1]湖北省地质科学研究院,武汉430034 [2]湖北省硒生态环境效应检测中心,武汉430034 [3]中化农业(临沂)研发中心有限公司,山东临沂276000 [4]长江大学农学院,湖北荆州434025

出　　处：《中国农学通报》2024年第13期113-120,共8页Chinese Agricultural Science Bulletin

基　　金：中化农业重点研发项目“蔬菜大棚土壤改良技术及应用研究”(072018017F);湖北省地质局项目“湖北省土地质量生态地球化学评价与监测预警关键技术研究”(KJ2022-2)。

摘　　要：为明确山东省临沂市草莓根腐病病原菌的种类及其生物学特性,采用组织分离法获得菌株ZHG01,经形态学特征及rDNA-ITS序列分析对菌株进行鉴定,通过研究不同培养环境对菌丝生长的影响探究菌株的生物学特性。结果表明,菌株ZH-G01平皿菌落正面呈白色丝绒状,未产生色素;背面略微发黄,分生孢子5个细胞,纺锤状,中心3个褐色异色胞,顶部具有3~4根附属丝;菌株ZH-G01 ITS序列与GenBank中新棒状拟盘多毛孢(Neopestalotiopsis clavispora)相似性达99%以上,结合形态学与分子生物学特征将菌株ZH-G01鉴定为新棒状拟盘多毛孢(登录号为MN559410)。菌株ZH-G01伤根接种后30 d草莓植株地上部萎蔫,中柱中心和不定根呈褐色,根部病组织可再次分离菌株ZH-G01。该菌株在25℃、pH 4.0~10.0条件下,培养于PDA、胡萝卜琼脂培养基中长势较好;光照对菌丝生长无显著影响;最适碳源为可溶性淀粉,对木糖醇利用效果最差;最适氮源为酵母膏,对尿素的利用效果最差。In order to clarify the pathogen and the biological characteristics of strawberry root rot disease occurring in Linyi City,Shandong Province,the strain ZH-G01 was obtained by using tissue isolation method.The strain was identified based on morphological characteristics and rDNA-internal transcribed spacer(ITS)analysis,and the biological characteristics of the strain were explored by studying the effects of different culture environments on mycelium growth.The results showed that colonies of strain ZH-G01 were white villous and the back was pale yellow.The conidia consisting of five cells were spindles with three or four hyaline appendages and three middle brown cells.The ITS sequence of strain ZH-G01 had over 99%homology with that of Neopestalotiopsis clavispora isolates in GenBank.The pathogen was identified as N.clavispora(GenBank accession No.MN559410).The shoot of strawberry plants wilted 30 days after inoculation of damaged roots of strain ZH-G01,the center of the stele and the adventitious roots were brown,and the diseased tissues of roots could be isolated again.The strain grew well in PDA and carrot agar at 25℃and pH range of 4.0-10.0.Light had no significant effect on the growth of mycelium.Soluble starch was the optimum carbon source,and xylitol utilization was the worst.Yeast extract was the optimum nitrogen source,and urea utilization was the worst.

关 键 词：草莓根腐病 新棒状拟盘多毛孢 鉴定 生物学特性 培养环境 菌丝生长 

分 类 号：Q93-3[生物学—微生物学]