多芯光纤荧光定量检测耦合系统设计  

Design of coupling system for fluorescence quantitative detection of multi-core optical fiber

作　　者：孙响 孔梅[1] 赵静涛 张荣[1] SUN Xiang;KONG Mei;ZHAO Jingtao;ZHANG Rong(School of Physics,Changchun University of Science and Technology,Changchun 130022,China)

机构地区：[1]长春理工大学物理学院,长春130022

出　　处：《光学技术》2024年第3期368-373,共6页Optical Technique

摘　　要：为提高多芯光纤荧光检测灵敏度,本文基于多芯光纤的空间传导理论模型,分析了多芯光纤的数量、激发光纤纤芯半径、接收光纤纤芯半径以及它们的数值孔径等对多芯光纤耦合效率的影响。针对提高耦合效率,设计和优化了耦合系统中非球面透镜的曲率半径和各面间距,基于分析设计优化的参数构建多芯光纤荧光定量检测系统,并对不同浓度梯度的罗丹明6G溶液进行定量测试,与多芯直接耦合对比,文章设计的多芯光纤耦合系统其激发光和发射光耦合效率由低于10%,提升了80.171%和95.314%,最低检测浓度低至2.08759551×10^(-9)nM/L,此浓度下荧光光强值较直接耦合提升了4.5倍。进一步验证了多芯光纤耦合系统射的可行性。说明通过增加光学耦合系统能有效提高光纤式荧光定量检测灵敏度及能够满足应用需求。To improve the fluorescence detection sensitivity of multi-core fiber,based on the spatial conduction theory model of multi-core fiber,the effects of the number of multi-core fiber,the core radius of excitation fiber,the core radius of receiving fiber and their numerical aperture on the coupling efficiency of multi-core fiber are analyzed.To improve the coupling efficiency,the curvature radius and spacing between non-spherical lenses in the coupling system are designed and optimized.Based on the analysis and optimization of the parameters,a multi-core optical fiber fluorescence quantitative detection system is constructed.Quantitative tests are conducted on different concentration gradients of Rhodamine 6G solution.Compared with direct coupling,the designed multi-core optical fiber coupling system shows an increase of 80.171% and 95.314% in excitation and emission light coupling efficiency,respectively,starting from below 10%.The lowest detection concentration is reduced to 2.08759551×10^(-9)nM/L,and the fluorescence intensity at this concentration is increased by 4.5 times compared to direct coupling.The feasibility of the multi-core optical fiber coupling system is further validated,demonstrating that increasing the optical coupling system can effectively improve the sensitivity of fiber-optic fluorescence quantitative detection and meet application requirements.

关 键 词：生物光学 光纤 多芯光纤 荧光定量检测系统 耦合系统 耦合效率 

分 类 号：O439[机械工程—光学工程]