RhD抗原表达强度对输血相容性检测低值阳性质控品制备的影响  

Effect of RhD antigen expression intensity on preparation of low positive quality control products for transfusion compatibility test

作　　者：李璐 魏俊杰 孙晓琳 吴伟鑫 刘瑞琪 刘海云 张印则 LI Lu;WEI Junjie;SUN Xiaolin;WU Weixin;LIU Ruiqi;LIU Haiyun;ZHANG Yinze(Department of Transfusion Medicine,Shenzhen University General Hospital,Shenzhen 518000,China)

机构地区：[1]深圳大学总医院输血科,广东深圳518000

出　　处：《中国输血杂志》2024年第6期690-693,共4页Chinese Journal of Blood Transfusion

基　　金：深圳市南山区卫生科技项目(NS 2021164)。

摘　　要：目的通过血清学结果比较RhD抗原表达强度差异,确定低值阳性质控品弱凝集强度的基准红细胞。方法以1500倍稀释抗-D分型试剂,使用微柱凝胶法对RhD(+)红细胞进行检测,挑选RhD抗原表达强度弱和强的标本分别为低值阳性质控品弱凝集强度的基准红细胞并进行验证。结果使用稀释后抗-D分型试剂对10份RhD(+)红细胞进行检测,其中8份凝集强度为1+、2份为±。以凝集强度呈1+的红细胞为基准,确定其凝集强度呈1+时抗-D分型试剂倍比稀释的最大稀释倍数,作为低值阳性质控品制备标准,则其与RhD抗原表达强度低的红细胞反应凝集强度极弱呈±,难以保障其控制界限性质稳定。以凝集强度呈±的红细胞为基准,重新确定其凝集强度呈1+时抗-D分型试剂倍比稀释的最大稀释倍数,作为低值阳性质控品制备标准,结果符合质控品设置要求。结论以RhD抗原表达强度低的红细胞为基准,设置低值阳性质控品弱凝集强度,可避免质控品因靶值较低而造成失控。Objective To determine the reference red blood cells with weak agglutination intensity of low positive quality control products by comparing RhD antigen expression intensity difference according to the serological results.Methods The RhD(+)red blood cells were detected by microcolumn gel method with 1500 times diluted anti-D typing reagent.The samples with weak and strong RhD antigen expression intensity were selected as the reference red blood cells for weak agglutination intensity of low positive quality control products,and verification was performed.Results Ten RhD(+)red blood cells were detected with diluted anti-D typing reagent,of which 8 were 1+and 2 were±.Red blood cells with agglutination intensity of 1+were used as the benchmark to determine the maximum dilution ratio of anti-D typing reagent when their agglutination intensity was 1+.As the preparation standard of low positive quality control products,the agglutination intensity of red blood cells with low RhD antigen expression intensity was extremely weak±,which was difficult to ensure the stability of its control limit properties.Based on red blood cells with agglutination intensity of±,the maximum dilution ratio of anti-D typing reagent with agglutination intensity of 1+was re-determined as the preparation standard of low positive quality control products,and the results met the requirements of quality control product setting.Conclusion Using red blood cells with low RhD antigen expression intensity as the benchmark to set the weak agglutination intensity of the low positive quality control products can avoid the loss of control due to the low target value.

关 键 词：RHD抗原 低值阳性 输血相容性检测 室内质控品 

分 类 号：R457.11[医药卫生—治疗学]