针灸对子宫内膜异位症大鼠异位内膜组织UHRF1和DNMT1的影响  

Effects of acupuncture and moxibustion on UHRF1 and DNMT1 in ectopic endometrium of rats with endometriosis

作　　者：李铭旸 王延文 邵燕婷 孙祎纯 胡佳玮 高源 李语然 吴楚婷 张春雁 LI Mingyang;WANG Yanwen;SHAO Yanting;SUN Yichun;HU Jiawei;GAO Yuan;LI Yuran;WU Chuting;ZHANG Chunyan(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Shanghai Minhang District Meilong Community Health Service Center,Shanghai 201108,China;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China [2]Shanghai Minhang District Meilong Community Health Service Center,Shanghai 201108,China [3]Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

出　　处：《Journal of Acupuncture and Tuina Science》2024年第3期204-213,共10页针灸推拿医学（英文版）

基　　金：上海市2022年度“科技创新行动计划”启明星培育-扬帆专项,No.22YF1444200;上海市卫生健康委员会卫生行业临床研究专项,No.202240054;上海中医药大学“杏林学者”;上海申康医院发展中心促进市级医院临床技能与临床创新能力三年行动计划项目,No.SHDC2020CR3093B.

摘　　要：目的:观察针灸对子宫内膜异位症(EMS)泛素样PHD和含环指结构域蛋白1(UHRF1)和DNA甲基转移酶1(DNMT1)的影响。方法:将40只Sprague-Dawley大鼠按体质量随机分为假手术组10只和造模组30只。造模组大鼠复制EMS模型后随机分为模型组、针灸组和孕酮组,每组10只。固定器固定各组大鼠。假手术组和模型组予以生理盐水灌胃;针灸组予以针刺血海和三阴交,艾灸关元和生理盐水灌胃;孕酮组给予地屈孕酮生理盐水混悬液灌胃。治疗28 d后,测量大鼠异位囊肿三径(长、宽及厚度)并计算体积,减掉干预前异位囊肿体积,取差值评估异位囊肿生长情况;实时荧光定量聚合酶链反应和免疫组织化学法检测正常子宫内膜、在位及异位内膜组织UHRF1、DNMT1 mRNA和蛋白水平。结果:针灸组和孕酮组异位囊肿体积差值无统计学差异(P>0.05),但均小于模型组异位囊肿体积差值(P<0.05)。模型组异位内膜组织UHRF1、DNMT1 mRNA和蛋白水平低于正常子宫内膜组织(P<0.05);模型组在位内膜组织DNMT1 mRNA以及UHRF1蛋白水平低于正常子宫内膜组织(P<0.05);针灸组异位内膜组织UHRF1 mRNA及蛋白水平、DNMT1蛋白水平高于模型组异位内膜组织(P<0.05),其中UHRF1 mRNA水平高于孕酮组(P<0.05);针灸组在位内膜组织DNMT1 mRNA水平高于模型组在位内膜组织(P<0.05);针灸组异位内膜组织UHRF1、DNMT1 mRNA和蛋白水平与正常子宫内膜比较无统计学差异(P>0.05)。结论:针灸可能通过上调异位内膜组织中UHRF1 mRNA和UHRF1、DNMT1蛋白水平,使其趋于正常子宫内膜水平,从而达到缩小异位囊肿体积,治疗大鼠子宫内膜异位症的效应。Objective:To observe the effects of acupuncture and moxibustion on ubiquitin-like containing PHD and RING finger domains 1(UHRF1)and DNA methyltransferase 1(DNMT1)in ectopic endometrium of rats with endometriosis(EMS).Methods:Forty Sprague-Dawley rats were randomly divided into a sham operation group with 10 rats and a model-building group with 30 rats according to body mass.EMS rat models were established in the model-building group and then were divided into a model group,an acupuncture and moxibustion group,and a progesterone group,with 10 rats in each group.All rats were fixed by a fixator.The sham operation group and the model group were given normal saline by gavage.The acupuncture and moxibustion group received acupuncture at Xuehai(SP10)and Sanyinjiao(SP6),moxibustion at Guanyuan(CV4),and gavage of normal saline.The progesterone group was given the mixed liquid made of dydrogesterone and normal saline by gavage.After 28 d of treatments,the three diameters(length,width,and height)of EMS rats’ectopic cysts were measured,the cyst volumes were calculated,the volumes before intervention were subtracted,and the difference values were used to evaluate the growth of ectopic cysts.UHRF1 and DNMT1 mRNA and protein levels in normal endometrium,eutopic endometrium,and ectopic endometrium were detected by real-time polymerase chain reaction and immunohistochemistry.Results:There was no significant difference in the ectopic cyst volume difference between the acupuncture and moxibustion group and the progesterone group(P>0.05),but they were smaller than that of the model group(P<0.05).The levels of UHRF1 and DNMT1 mRNA and protein in the ectopic endometrium of the model group were lower than those in the normal endometrium(P<0.05).The levels of DNMT1 mRNA and UHRF1 protein in the eutopic endometrium of the model group were lower than those in the normal endometrium(P<0.05).The levels of UHRF1 mRNA and protein and the level of DNMT1 protein in the ectopic endometrium of the acupuncture and moxibustion group were higher

关 键 词：针刺疗法 灸法 针灸疗法 子宫内膜异位症 泛素样PHD和含环指结构域蛋白1 DNA甲基转移酶1 大鼠 

分 类 号：R2-03[医药卫生—中医学]