ZOPOMED-60结核分枝杆菌显微扫描仪检测抗酸杆菌效果  

Evaluation of ZOPOMED-60 mycobacterium tuberculosis microscanner for detecting acid-fast bacilli in sputum smears

作　　者：董捷 唐柳生 杨小兵 黄丽玲 DONG Jie;TANG Liusheng;YANG Xiaobing;HUANG Liling(Medical Laboratory,Chest Hospital of Guangxi Zhuang Autonomous Region,Liuzhou 545005,Guangxi,China;Department of Science and Education,Chest Hospital of Guangxi Zhuang Autonomous Region,Liuzhou 545005,Guangxi,China)

机构地区：[1]广西壮族自治区胸科医院医学检验科,广西柳州545005 [2]广西壮族自治区胸科医院科教科,广西柳州5450051

出　　处：《中国现代医生》2024年第16期25-28,共4页China Modern Doctor

基　　金：广西医疗卫生适宜技术开发与推广应用项目(S2022042)。

摘　　要：目的 评价ZOPOMED-60结核分枝杆菌显微扫描仪在痰涂片查找抗酸杆菌的阅片效果。方法 收集2023年5月于广西壮族自治区胸科医院就诊患者的合格痰标本203份,分别采用萋-尼染色法和荧光染色法进行染色,通过人工镜检和ZOPOMED-60结核分枝杆菌显微扫描仪进行抗酸杆菌检测,以分枝杆菌液体培养为标准,计算不同方法查找抗酸杆菌的敏感度、特异性、阳性预测值、阴性预测值。结果 荧光染色人工阅片和仪器智能阅片的阳性率分别为12.3%和18.7%,萋-尼染色人工阅片和仪器智能阅片的阳性率分别为13.3%和17.7%,4种阅片检测结果差异无统计学意义(P>0.05)。以分枝杆菌液体培养结果为标准,荧光染色人工阅片和仪器智能阅片、萋-尼染色人工阅片和仪器智能阅片检测抗酸杆菌的敏感度分别为40.0%、45.0%、45.0%和53.3%,特异性分别为99.3%、92.3%、100.0%和97.2%,阳性预测值分别为96.0%、71.0%、100.0%和88.9%,阴性预测值分别为79.8%、80.0%、81.2%和83.2%,一致性均较好。结论 两种染色方法应用ZOPOMED-60结核分枝杆菌显微扫描仪检测抗酸杆菌的性能较好,值得推广应用。Objective To evaluate the effect of ZOPOMED-60 mycobacterium tuberculosis microscanner on detecting acid-fast bacilli in sputum smears.Methods A total of 203 qualified sputum specimens from patients treated in Chest Hospital of Guangxi Zhuang Autonomous Region in May 2023 were collected.The samples were stained by using Ziehl-Neelsen staining and fluorecent staining,respectively,and the acid-fast bacillus was detected by the manual microscopy and ZOPOMED-60 mycobacterium tuberculosis microscanner.The sensitivity,specificity,positive predictive value and negative predictive value of acid-fast bacillus were calculated using different methods based on liquid culture of mycobacterium.Results The positive rates of manual reading and instrument intelligent reading of fluorecent staining were 12.3% and 18.7%,respectively,and the positive rates of manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 13.3% and 17.7%,respectively.There was no significant difference between the four reading results(P>0.05).Based on the results of liquid culture of mycobacterium,sensitivity of manual reading and instrument intelligent reading of fluorecent staining and manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 40.0%,45.0%,45.0% and 53.3%,specificity were 99.3%,92.3%,100.0% and 97.2%,positive predictive values were 96.0%,71.0%,100.0% and 88.9%,and negative predictive values were 79.8%,80.0%,81.2% and 83.2%,respectively.The consistency was good.Conclusion The performance of ZOPOMED–60 mycobacterium tuberculosis microscanner in the detection of acid-fast bacilli by two staining methods is good,and it is worth popularizing.

关 键 词：显微扫描仪 抗酸杆菌 敏感度 特异性 一致性 

分 类 号：R446[医药卫生—诊断学]