不同LED光质对大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代组培苗生长的影响  

Effects of Different LED Light Quality on the Growth of Cymbidium hybridum‘Dafeng’×C.serratum var.goeringii‘Taiji Shengmei’F1 Seedlings

作　　者：张金龙 赵红梅 瞿素萍[2] 李枝林[1] 和凤美[1] 王玉英[1] ZHANG Jinlong;ZHAO Hongmei;QU Suping;LI Zhilin;HE Fengmei;WANG Yuying(College of Gardening and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201;Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《北方园艺》2024年第10期61-68,共8页Northern Horticulture

基　　金：云南省重大科技专项资助项目(202102AE090052);云南省基础研究资助项目(202001AT070101);云南省创新团队资助项目(202105AE160012)。

摘　　要：以大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代组培苗为试材,采用不同LED光质照射方法,研究了不同LED光质照射对大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代组培苗生长的影响,以期为兰花新品种选育和产业化种苗生产提供参考依据。结果表明:1)黄光(Y)照射下,植株最高、根最粗壮;红光(R)照射下植株根长最长;红蓝黄复合光(RBY)照射下,不定芽增殖系数最大,为2.03;红蓝绿复合光(RBG)照射下植株的叶长、叶宽、叶片数、茎粗、鲜质量效果最佳,分别为3.82 cm、4.36 mm、11.66片、7.97 mm、1.49 g。2)蓝光(B)照射下,可溶性蛋白质含量最高,为0.1157 mg·g^(-1);红蓝复合光(RB)照射下,可溶性糖含量最高;RBG照射下,叶绿素含量最高,为2.15 mg·g^(-1)。综上,与白色荧光相比,LED光质对大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1组培苗生长有促进作用;RBG处理促进植株生长和叶绿体色素合成,RBY照射有利于芽增殖,B处理促进蛋白质合成,RB处理促进可溶性糖积累;RGB可作为大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代组培苗生长的最佳光源。The F1 generation tissue culture seedlings of Cymbidium hybridum‘Dafeng’×C.serratum var.goeringii‘Taiji Shengmei’were used as test materials,and different LED light quality irradiation methods were used to study the effects of different LED light quality irradiation on the growth of F1 generation tissue culture seedlings of Cymbidium hybridum‘Dafeng’×C.serratum var.goeringii‘Taiji Shengmei’,in order to provide reference for the breeding of new varieties of orchids and the production of industrial seedlings.The results showed that,1)under yellow light(Y)irradiation,the plant was the highest and the root was the thickest.The root length of the plant was the longest under red light(R)irradiation.Under the irradiation of red,blue and yellow composite light(RBY),the proliferation coefficient of adventitious buds was the largest,which was 2.03.The leaf length,leaf width,leaf number,stem diameter and fresh weight of the plants under red,blue and green composite light(RBG)irradiation were the best,which were 3.82 cm,4.36 mm,11.66 pieces,7.97 mm and 1.49 g,respectively.2)Under blue light(B)irradiation,the soluble protein content was the highest,which was 0.1157 mg·g^(-1);under the red and blue compound light(RB)irradiation,the soluble sugar content was the highest;under RBG irradiation,the chlorophyll content was the highest,which was 2.15 mg·g^(-1).Based on the above,compared with white fluorescence,LED light quality could promote the growth of F1 tissue culture seedlings of Cymbidium hybridum‘Dafeng’×C.serratum var.goeringii‘Taiji Shengmei’.RBG treatment promoted plant growth and chloroplast pigment synthesis,RBY irradiation was beneficial to bud proliferation,B treatment promoted protein synthesis,and RB treatment promoted soluble sugar accumulation.RGB could be used as the best light source for the growth of F1 seedlings of Cymbidium hybridum‘Dafeng’×C.serratum var.goeringii‘Taiji Shengmei’.

关 键 词：LED光质 大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代 组织培养 生理指标 

分 类 号：S682.31[农业科学—观赏园艺]