蹄叶橐吾萃取物对金黄色葡萄球菌的抗菌消炎作用研究  

Study on the Antibacterial and Anti-inflammatory Effects of Ligulariafischeri Extract on Staphylococcus Aureus

作　　者：张春艳 郭明飞 乌仁高娃 吴丽娟 乌吉木 胥向红 刘万全 ZHANG Chun-yan;GUO Ming-fei;WUREN Gao-wa;WU Li-juan;WU Ji-mu;XU Xiang-hong;LIU Wan-quan(Inner Mongolia Key Laboratory of Human Genetic Disease Research,Basic Medical College,Chifeng University,Chifeng 024005,Inner Mongolia,China;Basic Medical College,Chifeng University,Chifeng 024005,Inner Mongolia,China;Basic Medical College,Qiqihar Medical University,Qiqihar 161006,Heilongjiang,China)

机构地区：[1]赤峰学院基础医学院内蒙古人类遗传病研究重点实验室,内蒙古赤峰024005 [2]赤峰学院基础医学院,内蒙古赤峰024005 [3]齐齐哈尔医学院基础医学院,黑龙江齐齐哈尔161006

出　　处：《医学信息》2024年第12期89-92,97,共5页Journal of Medical Information

基　　金：赤峰学院青年科研基金项目(编号:CFXYQNZR2230);内蒙古自治区自然科学青年基金项目(编号:2024QN08078)。

摘　　要：目的研究蹄叶橐吾醇化乙酸乙酯萃取物(EEOLF)对金黄色葡萄球菌的抗菌消炎作用。方法测定EEOLF对金黄色葡萄球菌的最小抑菌浓度(MIC);取正常培养的巨噬细胞RAW264.7为空白组,采用金黄色葡萄球菌作用于RAW264.7细胞建立体外实验模型(模型组)。细胞活/死染色观察EEOLF(剂量为5μg/ml)作用于模型组后细胞的损伤情况,ELISA法测定EEOLF(剂量为1.25、2.5及5μg/ml)作用于模型组后IL-1β及TNF-α的释放量。结果EEOLF对金黄色葡萄球菌生长有抑制作用,最小抑菌浓度为1300μg/ml;当金黄色葡萄球菌作用于RAW264.7细胞后,细胞发生严重损伤,同时IL-1β及TNF-α的分泌量增加(P<0.05)。对金黄色葡萄球菌感染的细胞给药后,药物浓度为1.25μg/ml和2.5μg/ml时,炎症因子的分泌量没有明显减少(P>0.05),当药物浓度为5μg/ml时,炎性因子L-1β及TNF-α的分泌量下降(P<0.05),同时细胞损伤情况改善。结论EEOLF能够抑制金黄色葡萄球菌生长,也能缓解金黄色葡萄球菌引起的炎症反应,其抗菌消炎作用值得深入研究。Objective To study the antibacterial and anti-inflammatory effects of ethanolic extract of ligulariafischeri(EEOLF)on Staphylococcus aureus.Methods The minimum inhibitory concentration(MIC)of EEOLF against Staphylococcus aureus was determined.Normal cultured macrophages RAW264.7 were taken as blank group,and Staphylococcus aureus was applied to RAW264.7 cells to establish cell inflammation model group in vitro(model group).The cell damage after EEOLF(5μg/ml)in the model group was detected by living/dead staining,and the release of IL-1βand TNF-αafter EEOLF(1.25,2.5 and 5μg/ml)in the model group was determined by ELISA.Results EEOLF could inhibit the growth of Staphylococcus aureus,and the MIC was 1300μg/ml.When Staphylococcus aureus acted on RAW264.7 cells,the cells were severely damaged,and the secretion of IL-1βand TNF-αwas increased(P<0.05).When the drug concentration was 1.25μg/ml and 2.5μg/ml,the secretion of inflammatory factors was not significantly reduced(P>0.05).When the drug concentration was 5μg/ml,the secretion of inflammatory factors L-1βand TNF-αwas decreased(P<0.05),and the cell damage was improved.Conclusion EEOLF can inhibit the growth of Staphylococcus aureus and alleviate the inflammatory response caused by Staphylococcus aureus,and its antibacterial and anti-inflammatory effects are worthy for further study.

关 键 词：蹄叶橐吾醇化乙酸乙酯萃取物 金黄色葡萄球菌 抗菌消炎 

分 类 号：R259[医药卫生—中西医结合]