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作 者:余昕 陈法志 刘忠 陈志伟 戢小梅 李秀丽 YU Xin;CHEN Fa-zhi;LIU Zhong;CHEN Zhi-wei;JI Xiao-mei;LI Xiu-li(Institute of Forestry and Fruit Tree,Wuhan Academy of Agricultural Sciences,Wuhan 430075,China)
机构地区:[1]武汉市农业科学院林业果树研究所,武汉430075
出 处:《湖北农业科学》2024年第6期116-120,共5页Hubei Agricultural Sciences
基 金:湖北省自然科学基金青年项目(2019CFB351);湖北省技术创新专项重大项目(农业领域)(2018ABA073);中央引导地方科技专项(2022BGE228)。
摘 要:以13个代表性牡丹(Paeonia suffruticosa Andr.)品种为试材,利用电导率法、Logistic方程研究高温半致死温度(LT50),以期评价牡丹种质资源的耐热性。结果表明,在逐步高温处理过程中,13个牡丹品种的细胞伤害率呈典型的S形曲线增长趋势,经显著性检验符合Logistic方程。13个牡丹品种的LT50为51.31~59.73℃,其中B24的LT50最高,为59.73℃,而海黄的LT50最低,为51.31℃。利用有序样品最优分割聚类法,将13个牡丹品种分为3个类群,B24、太平红、B10、凤丹荷和凤丹白属于耐热类型,LT50平均值为57.70℃;香玉、莲鹤、五星玉、岛锦和垫江红属于中度耐热类型,LT50平均值为56.32℃;ZB1、泼墨紫和海黄属于不耐热类型,LT50平均值为53.32℃。In order to evaluate the heat resistance of tree peony(Paeonia suffruticosa Andr.)germplasm resources,13 representative tree peony cultivars were selected as experimental materials,and the semi-lethal high temperature(LT50)was studied by the conductivity method and Logistic equation.The results showed that with the treating temperatures higher step-by-step,the cell damage rate of 13 tree peony cultivars showed a typical S curve increasing trend,and the significance test conformed to the Logistic equation.The LT50of 13 tree peony cultivars ranged from 51.31 ℃ to 59.73 ℃,among which B24 had the highest LT50of 59.73 ℃,while Haihuang had the lowest LT50of 51.31 ℃.Based on sequential clustering method,13 tree peony cultivars could be divided into 3 groups:B24,Taipinghong,B10,Fengdanhe and Fengdanbai belonged to the heat resistant type,with an average LT50of 57.70 ℃;Xiangyu,Lianhe,Wuxingyu,Daojin and Dianjianghong belonged to the moderate heat resistant type,with an average LT50of 56.32 ℃;ZB1,Pomozi and Haihuang belonged to the heat sensitive type,with an average LT50of 53.32 ℃.
关 键 词:牡丹(Paeonia suffruticosa Andr.) 细胞伤害率 LOGISTIC方程 高温半致死温度 耐热性
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