基于非靶向代谢组学挖掘两种日粮模式下蛋鸡回肠代谢物的差异  

Exploring the differences in ileal metabolites of laying hens under two different dietary patterns based on non targeted metabolomics

作　　者：张应胡 秦绪光 马丽[1] 王皓杰 胡玲[1] 田光明[1] ZHANG Ying-hu;QIN Xu-guang;MA Li;WANG Hao-jie;HU Ling;TIAN Guang-ming(College of Animal Science and Technology,Yangtze University,Jingzhou 434025,Hubei,China;Rizhao Animal Disease Prevention and Control Center,Rizhao 276826,Shandong,China)

机构地区：[1]长江大学动物科学技术学院,湖北荆州434025 [2]日照市动物疫病预防控制中心,山东日照276826

出　　处：《湖北农业科学》2024年第6期213-217,227,共6页Hubei Agricultural Sciences

基　　金：农村农业部畜禽细菌病防治制剂创制重点实验室开放课题(KLPCAAB-2022-03)。

摘　　要：为探究饲喂基础日粮(CON)和含5.0%益生菌发酵饲料(PFF)2种日粮条件下蛋鸡回肠内容物代谢物差异,采用液相色谱-质谱和非靶向代谢组学相结合的方法,检测2种日粮条件下蛋鸡回肠中代谢产物,通过差异代谢物筛选和KEGG富集分析,筛选出蛋鸡回肠中的差异代谢物及代谢通路。结果表明,与CON组相比,PFF组中胆酸、牛磺鹅去氧胆酸、鹅去氧胆酸等胆汁酸含量显著降低,异亮氨酸-亮氨酸-甘氨酸-丙氨酸、苯丙氨酸-谷氨酸-缬氨酸-谷氨酸、亮氨酸-酪氨酸-苏氨酸-赖氨酸等小肽含量显著增加;KEGG通路富集分析表明,代谢物主要与胆汁分泌、初级胆汁酸生物合成等通路相关,初级胆汁酸在宿主脂质和脂溶性物质的消化代谢、抗氧化状态、胆固醇代谢和宿主-微生物相互作用中发挥重要作用。蛋鸡日粮中添加益生菌发酵饲料可通过改变肠道代谢产物来改善肠道吸收功能。To investigate the differences in metabolites in the ileum contents of laying hens fed with two different dietary conditions:basal diet(CON)and fermented feed containing 5.0% probiotics(PFF),a combination of liquid chromatography-mass spectrometry and non targeted metabolomics was used to detect metabolites in the ileum of laying hens under two different dietary conditions.Differential metabolite screening and KEGG enrichment analysis were performed to screen for differential metabolites and metabolic pathways in the ileum of laying hens.The results showed that compared with the CON group,the content of bile acids such as bile acid,taurine goose deoxycholic acid,and goose deoxycholic acid in the PFF group was significantly reduced,while the content of small peptides such as isoleucine-leucine-glycine-alanine,phenylalanine-glutamate-valine-glutamate,and leucine-tyrosine-threonine-lysine was significantly increased;KEGG pathway enrichment analysis showed that metabolites were mainly related to pathways such as bile secretion and primary bile acid biosynthesis.Primary bile acids played important roles in the digestion and metabolism of host lipids and lipophilic substances,antioxidant status,cholesterol metabolism,and host-microbial interactions.Adding probiotic fermented feed to the diet of laying hens could improve intestinal absorption function by altering intestinal metabolites.

关 键 词：发酵饲料 蛋鸡 回肠代谢产物 非靶向代谢组学 

分 类 号：S831.5[农业科学—畜牧学]