聚鸟氨酸修饰电极对丹皮酚的灵敏测定  

Sensitive Determination of Paeonol in Traditional Chinese Medicine by Polyornithine Modified Electrode

作　　者：孙章华 王苍 庄润梦 陈美凤 SUN Zhang-hua;WANG Cang;ZHUANG Run-meng;CHEN Mei-feng(Environmental Monitoring Central Station of Heze,Shandong Heze 274000;Heze Maternal and Child Health Care Hospital,Shandong Heze 274015;China College of Chemistry and Chemical Engineering of Heze University,Shandong Heze 274015,China)

机构地区：[1]山东省菏泽生态环境监测中心,山东菏泽274000 [2]菏泽市牡丹区妇幼保健院,山东菏泽274015 [3]菏泽学院化学化工学院,山东菏泽274015

出　　处：《广州化工》2024年第4期57-59,共3页GuangZhou Chemical Industry

基　　金：山东省高等学校科技计划项目(J17KB62)。

摘　　要：建立了灵敏测定Pae的电化学新方法。采用电化学聚合法制备了聚鸟氨酸(Ornithine,PO)修饰电极(modified glass electrode,GME)。优化了聚合pH值、扫描速率、电压、扫描段数等实验条件。建立了Pae的浓度(c)内与其氧化峰电流(i_(pa))的关系,即在8.0×10^(-8) mol·L^(-1)~8.0×10^(-5) mol·L^(-1):i_(pa)=3.94×10^(-7)+0.086c(mol·L^(-1)),相关系数R=0.9932,最低检出限为8.0×10^(-9) mol·L^(-1)。该方法经济、简便、快速,线性范围宽,灵敏度高,可用于丹皮酚软膏中Pae的测定,测定结果与LC/MS方法一致,结果令人满意。An electrochemical method was established for the determination of Pae.Ornithine modified electrode(PO/GME)was prepared by electrochemical polymerization.The experimental conditions,including the electrochemical polymerization pH value of the PO solution,scanning rate,voltage,sweep segments were optimized.A linear relationship between the concentration(C)of Pae and its oxidation peak current(i_(pa))on the PO/GME in the range of 8.0×10^(-8) mol·L^(-1)-8.0×10^(-5) mol·L^(-1) was established,and its linear regression equation is as follows:i_(pa)=3.94×10^(-7)+0.086c(mol·L^(-1)),the correlation coefficient R=0.9932,the lowest detection limit was 8.0×10^(-9) mol·L^(-1).The method used is economical,simple and rapid with a wide linear range and high sensitivity,and the sensor can be applied to the determination of Pae in paeonol ointment.The results obtained by this method and LC-MS are in good agreement and the results were satisfactory.

关 键 词：鸟氨酸 电化学聚合 修饰电极 丹皮酚 

分 类 号：R927.2[医药卫生—药学]