电针夹脊穴对腰椎间盘退变兔髓核细胞Cav-1和p38 MAPK蛋白表达的影响  

Effects of Electroacupuncture Jiaji Acupoint on the Protein Expression of Cav-1 and p38 MAPK in Nucleus Pulposus Cells of Lumbar Intervertebral Disc Degeneration in Rabbits

作　　者：边清灵 邹璟[2] 黄国付 BIAN Qingling;ZOU Jing;HUANG Guofu(College of Acupuncture and Orthopedics,Hubei University of Chine Medicine,WuhanHubei430065,China)

机构地区：[1]湖北中医药大学针灸骨伤学院,湖北武汉430065 [2]武汉市中西医结合医院针灸科 [3]深圳市龙岗区妇幼保健院中医科

出　　处：《联勤军事医学》2024年第4期271-276,共6页Military Medicine of Joint Logistics

基　　金：国家自然科学基金项目(82074517)。

摘　　要：目的 通过观察电针夹脊穴对腰椎间盘退变(intervertebral disc degeneration, IDD)兔髓核细胞中小窝蛋白1(caveolin 1,Cav-1)及p38丝裂原活化蛋白激酶(p38 mitogen-activated protein kinase, p38 MAPK)蛋白表达的影响,探讨电针延缓IDD髓核细胞衰老的作用机制。方法 采用随机数字表法将25只雄性新西兰大白兔随机分为正常组、假模型组、模型组、模型+电针组及模型+假电针组,每组各5只。造模方式采用加压器对L_4~L_5椎间盘进行200 N压力持续轴向加压28天,通过磁共振成像(magnetic resonance imaging, MRI)判断是否造模成功。正常组兔予以自然喂养,不予任何干预;模型组兔使用加压器对L_4~L_5椎间盘加压28天,造模成功后不作任何治疗;假模型组兔同模型组造模方法,保留加压装置及克氏针但不完成椎体加压及干预治疗;模型+电针组兔在模型组的基础上,自造模成功后第1天起予以双侧L_4~L_5夹脊穴电针治疗,电针强度为1~2mA,每次20 min, 1次/天,6天为1疗程,每个疗程间隔1天,共4个疗程;模型+假电针组兔同模型+电针组方法,但在针刺夹脊穴时仅接上电针不接通电流。实验周期结束后,观察各组兔一般情况、MRI下椎间盘信号强度并进行Pfirrmann分级,并采用Western blot法检测椎间盘髓核细胞中Cav-1及p38 MAPK蛋白表达。结果 与正常组兔比较,假模型组兔的一般情况无明显变化,MRI下椎间盘信号差异较小;模型组兔一般情况变差,MRI下椎间盘信号为黑色低信号表现;模型+电针组兔一般情况明显缓解改善,MRI下椎间盘信号增强呈灰-白信号表现,质地更均匀;模型+假电针组兔一般情况较差,无明显改善,MRI下椎间盘呈灰黑色信号,未见明显信号改善。同正常组兔比较,假模型组兔椎间盘Pfirrmann评分、椎间盘髓核细胞Cav-1蛋白表达、p38 MAPK蛋白表达差异无统计学意义(P>0.05),模型组兔椎间盘Pfirrmann评分、椎间盘髓核细胞Cav-1蛋白表�Objective To observe the effects of electroacupuncture Jiaji acupoint on the expression of caveolin 1(Cav-1) and p38 mitogen-activated protein kinase(p38 MAPK) protein in the nucleus pulposus cells of rabbits with lumbar intervertebral disc degeneration(IDD) and to investigate the mechanism of electroacupuncture in delaying the aging of the nucleus pulposus cells of IDD.Methods A total of 25 male New Zealand rabbits were randomly divided into normal group, sham model group, model group, model+ electroacupuncture group and model+ sham electroacupuncture group, with 5 rabbits in each by random number table method. The L_4-L_5 intervertebral disc was pressurized with 200 N pressure for 28 days. Magnetic resonance imaging(MRI) was used to determine whether the model was successfully established. Rabbits in the normal group were fed naturally without any intervention;the rabbits in the model group without receiving any treatment after the model was successfully established by pressurizing the L_4-L_5 intervertebral discs for 28 days;in the sham model group, on the basis of molding method in the model group, the compression device and Kirschner wire were retained, but the vertebral compression and intervention treatment were not completed;in the model + electroacupuncture group, on the basis of the treatment in the model group, the rabbits were treated with bilateral L_4-L_5 Jiaji electroacupuncture from the first day after successful modeling, the intensity of electroacupuncture was 1-2 mA, with 20 min each time, once a day, 6 days as a course of treatment, and each course was 1 day apart, totally 4 courses;in the model + sham electroacupuncture group and model + electroacupuncture group, electroacupuncture was connected after acupuncture at Jiaji acupoint, but no electricity was connected. After the end of the experimental period, the general condition and MRI lower disc signal strength were observed, and the Pfirrmann scoring was performed, the protein expression of Cav-1 and p38 MAPK in intervertebral disc nucleus

关 键 词：电针夹脊穴 小窝蛋白1 P38丝裂原活化蛋白激酶 椎间盘退变 细胞衰老 

分 类 号：R245.9[医药卫生—针灸推拿学]