基于网络药理学探究北五味子治疗缺血性脑卒中机制  

Exploration of the Mechanism of Schisandra Chinensis in Treating Ischemic Stroke Based on Network Pharmacology

作　　者：李显伟[1] 张宇[1] 黄鑫[1] Li Xianwei;Zhang Yu;Huang Xin(Department of Neurosurgery,Daqing Longnan Hospital,Daqing,Heilongjiang,163000,China)

机构地区：[1]大庆龙南医院神经外科,黑龙江大庆163000

出　　处：《黑龙江医学》2024年第12期1433-1436,共4页Heilongjiang Medical Journal

摘　　要：目的:探究北五味子治疗缺血性脑卒中的有效活性物质和具体机制。方法:通过中药系统药理学技术平台筛选出北五味子的有效活性成分,应用Swiss target prediction数据库获得有效活性物质的作用靶点;通过人类基因数据库和在线人类孟德尔遗传数据库获得缺血性脑卒中的作用靶点;通过韦恩图获得共同靶点,借助String数据库进行蛋白质互作网络的建立;通过DAVID数据库进行基因本体(GO)分析获得生物学过程条目、分子功能条目和细胞组成条目;通过基因组百科全书(KEGG)通路富集分析,获得北五味子发挥作用的潜在作用机制;通过Cytoscape 3.8.2软件建立北五味子有效成分-靶点-通路网络。结果:北五味子具有7种治疗缺血性脑卒中的有效成分,18个核心靶点;通过GO分析发现北五味子治疗缺血性脑卒中涉及220个生物学过程条目,27个细胞组成条件,34个分子功能条目;通过KEGG通路富集分析发现北五味子发挥治疗缺血性脑卒中涉及到VEGF信号通路、PI3K-Akt信号通路、脂质与动脉粥样硬化通路和TNF信号通路等;北五味子治疗缺血性脑卒中网络涉及到35个节点、184条边,平均相邻节点数目为10.514,网络中心度为0.309。结论:北五味子有效成分、多靶点、多通路起到防治缺血性脑卒中的作用。本次研究预测的北五味子防治缺血性脑卒中潜在机制可为开展实验研究及临床应用提供依据。Objective:To explore the effective active substances and specific mechanisms of Schisandra chinensis in the treatment of ischemic stroke.Methods:The effective ingredients of Schisandra chinensis were screened using the traditional Chinese medicine system pharmacology database and analysis platform,and the action targets of effective active substances were obtained using the Swiss target prediction database.The related targets of ischemic stroke were obtained from GeneCards and the online Mendelian Genetic Database.The common targets were obtained through Wayne diagrams and the protein interaction network network was established using String database.The biological process items,molecular function items,and cell components items were obtained through GO analysis using the DAVID database.The potential mechanisms of Schisandra chinensis were obtained through KEGG pathway enrichment analysis.The effective components-targets-pathways network was established with Cytoscape 3.8.2 software.Results:Schisandra chinensis has 7 effective ingredients and 18 core targets for treating ischemic stroke.Through GO analysis,it was found that the treatment of ischemic stroke by Schisandra chinensis involved 220 biological process items,27 cell component items,and 34 molecular functional items.Through KEGG pathway enrichment analysis,it is found that the treatment of ischemic stroke by Schisandra chinensis involved VEGF signaling pathway,PI3K-Akt signaling pathway,lipid and atherosclerosis signaling pathway and TNF signaling pathway.The network of Schisandra chinensis for treating ischemic stroke involved 35 nodes and 184 edges,with an average number of adjacent nodes of 10.514 and a network centrality of 0.309.Conclusion:Schisandra chinensis plays a role in treating ischemic stroke with multiple active ingredients,targets,and pathways.The potential mechanism of Schisandra chinensis in treating ischemic stroke predicted can be used as a basis for experimental research and clinical application.

关 键 词：北五味子 网络药理学 缺血性脑卒中 

分 类 号：R285[医药卫生—中药学]