A new tetracyclic triterpenoid from endophytic fungus Fusarium sporotrichioides  

作　　者：Yajing Wang Chunyue Liu Yihui Yang Chang Li Yuehu Pei 

机构地区：[1]Department of Medicinal Chemistry and Natural Medicine Chemistry,College of Pharmacy,Harbin Medical University,Harbin 150081,China

出　　处：《Chinese Herbal Medicines》2024年第2期231-234,共4页中草药（英文版）

基　　金：funded by Outstanding Youth Foundation of Heilongjiang province(NO.YQ2021H009).

摘　　要：Objective: To isolate bioactive compounds from the endophytic fungus Fusarium sporotrichioides isolated from Rauwolfia yunnanensis, and investigate their pharmacological activities.Methods: The chemical constituents were isolated and purified by combining with ODS column chromatography, silica gel column chromatography and by performing semipreparative HPLC. Their structures were established on the basis of 1D NMR(1H-NMR and13C-NMR) and 2D NMR(1H–1H COSY,HSQC, HMBC and NOESY), as well as HRESIMS and comparison with literature data. In addition, the absolute configuration of compound 1 was determined by calculated ECD data.Results: One previously undescribed tetracyclic triterpenoid derivative, named as integracide L(1), 12aacetoxy-4,4-dimethyl-24-methylene-5a-cholesta-8,14-diene-2a,3β,11β-triol(2), 12a-acetoxy-4,4-dime thyl-24-methylene-5a-cholesta-8-momoene-2a,3β,11β-triol(3), 12a-acetoxy-4,4-dimethyl-24-methy lene-5a-cholesta-8,14-diene-3β,11β-triol(4), and 12a-acetoxy-4,4-dimethyl-24-methylene-5acholesta-8-momoene-3β,11β-triol(5) were isolated from F. sporotrichioide. Moreover, compound 1 was rare tetracyclic triterpenoid with single methyl replacement at C-4 position.Conclusion: Compound 1 was a new tetracyclic triterpenoid isolated from the endophytic fungus F.sporotrichioides. In addition, compound 2 could inhibit the growth of three different human cancer cells significantly. Compounds 3 and 5 were found to possess better cytotoxic activities on Hep G-2 cells than the other compounds, with IC50values of(2.8 ± 0.1) and(6.3 ± 0.3) μmol/L respectively.

关 键 词：antitumor Fusarium sporotrichioides integracide L tetracyclic triterpenoid 12a-acetoxy-4 4-dimethyl-24-methylene5a-cholesta-814-diene-2a 3b 11b-triol 12a-acetoxy-4 4-dimethyl-24-methylene5a-cholesta-8-momoene-2a 3b 11b-triol 12a-acetoxy-4 4-dimethyl-24-methylene5a-cholesta-8-momoene-3b 11b-triol 

分 类 号：R284[医药卫生—中药学]