Chemical profiling of principle active and toxic constituents in herbs containing aristolochic acids  

作　　者：Lijun Yue Kaijun Yang Feng Jiang Shuai Dong Kang Yang Dan Zhu 

机构地区：[1]School of Pharmacy,Minzu University of China,Beijing 100081,China [2]Key Laboratory of Ethnomedicine Ministry of Education,Minzu University of China,Beijing 100081,China [3]Hospital of Minzu University of China,Beijing 100081,China

出　　处：《Chinese Herbal Medicines》2024年第2期293-300,共8页中草药（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81603076);Minzu University of China(No.2021MDYY53).

摘　　要：Objective:To clear the amounts of the principal active/toxic components in herbs containing aristolochic acids(HCAAs),which are still used as medicine and/or seasoning in many ethnic minority areas of China.Methods:In this study,six major active and toxic components in HCAAs were extracted with ultrasonic extraction.With 6-O-methyl guanosine as internal standard,the target compounds were analyzed qualitatively and quantitatively by using ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-ESI-MS/MS)with multiple reaction monitoringinformation dependent acquisition-enhanced production ion scanning mode(MRM-IDA-EPI)combined with dynamic background subtraction(DBS)function.Results:The method showed good linearity in the linear range of the six analytes.The limit range of detection was from 0.01 ng/mL to 0.27 ng/mL.All of the detection repeatability,extraction repeatability and accuracy of the method were good.After extraction,the samples remained stable at 15℃ within 24 h.Six analytes were all found in samples except aristolactam(AL)in sample 2,and the contents varied greatly.The contents of these compounds decreased in fruits,leaves and stems of Aristolochia delavayi successively.Conclusion:This method has the advantages of less sample dosage,simple operation,short analysis cycle,high sensitivity,specificity and accuracy.It laid a good foundation for guiding the safety of HCAAs,the indepth study of pharmacological and toxicological effects and the scientific and standardized processing and compatibility of HCAAs.

关 键 词：aristolactam aristolochic acids chemical profiling herbs containing aristolochic acids ultrahigh performance liquid chromatography-electrospray ionizationtandem mass spectrometry(UPLC-ESI-MS/MS) 

分 类 号：R284.1[医药卫生—中药学]