杨树响应腐皮镰刀菌侵染的转录组学分析  

Transcriptome Analysis of Populus in Response to Fusarium solani(Mart.)Sacc Inoculation

作　　者：郝心怡 王哲舒 范志斌 王丽娟[1,2] HAO Xin-yi;WANG Zhe-shu;FAN Zhi-bin;WANG Li-juan(State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Collaborative Innovation Center of Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]中国林业科学研究院林业研究所,林木遗传育种全国重点实验室,国家林业和草原局林木培育重点实验室,北京100091 [2]南京林业大学,南方现代林业协同创新中心,江苏南京210037

出　　处：《林业科学研究》2024年第3期24-36,共13页Forest Research

基　　金：国家重点研发计划(2021YFD2200201);农业生物育种重大项目(2022ZD0401505)。

摘　　要：[目的]基于RNA-Seq测序技术,初步探究了杨树-腐皮镰刀菌互作过程中相关基因的表达、主要信号通路和代谢途径,筛选了杨树防御腐皮镰刀菌侵染的关键基因,为进一步揭示此类病害的分子机制奠定基础。[方法]以生长2个月的银腺杨84K为材料,用1×10^(7)个·mL^(-1)的腐皮镰刀菌孢子液侵染根系,于侵染0h(对照组)、48 h和72 h(接菌组)后取根组织进行转录组测序,挖掘杨树响应腐皮镰刀菌侵染的相关基因。[结果](1)与对照组0 h相比,侵染48 h和72 h分别检测到8 939个和8 246个DEGs(Differentially Expressed Genes)。(2)GO分析发现,差异表达基因主要富集在单一生物体过程、对刺激反应、碳水化合物代谢、生物调节和对激素响应等过程。(3)KEGG分析表明,糖酵解/糖异生、碳代谢、植物激素信号转导和苯丙烷生物合成等途径在病原菌侵染后发生显著变化。(4)杨树糖转运蛋白SWEETs家族中21个成员的表达受腐皮镰刀菌侵染诱导。乙烯受体ETR、乙烯信号通路主要基因EIN3、ERF1、ERF2上调表达,木质素合成关键酶基因CCR、4CL、C3’H、COMT表达量显著升高。[结论]杨树可能通过调节体内糖代谢和转运,激活乙烯信号通路,促进木质素积累、细胞壁增厚等策略,响应腐皮镰刀菌的侵染。[Objective]Based on RNA-sequencing,the differentially expressed genes,related signaling pathways and metabolic pathways in poplar responded to Fusarium solani(Mart.)Sacc inoculation were investigated,and the key genes involved in the interaction were screened.The present results will lay a foundation for further revealing the molecular mechanism underlying poplar resistance to F.solani(Mart.)Sacc.[Methods]The roots of 2-month-old seedlings of 84K were inoculated with 1×10^(7)spores·mL^(-1)of F.solani(Mart.)Sacc spore solution.Zero-hour(control group),48 h and 72 h(inoculated groups)after in-oculation,root tissues were taken for transcriptome sequencing to explore genes related to poplar re-sponse to F.solani(Mart.)Sacc infection.[Result](1)Compared with the control group,8939 and 8246 DEGs(Differentially Expressed Genes)were detected in 48 h and 72 h inoculated samples,respectively.(2)GO analysis found that the differentially expressed genes were mainly enriched in single-organism pro-cess,response to stimulus,carbohydrate metabolism process,biological regulation and response to hor-mone.(3)KEGG pathway enrichment analysis showed that the DEGs were significantly enriched in glyco-lysis/gluconeogenesis,carbon metabolism,plant hormone signal transduction and phenylpropanoid bio-synthesis.(4)The expressions of 21 genes in the SWEETs family of poplar were induced by F.solani(Mart.)Sacc.Ethylene receptor ETR,and the main components of the ethylene signaling pathway EIN3,ERF1,ERF2 were up-regulated.The expression levels of key enzyme genes involved in lignin synthesis including CCR,4CL,C3’H and COMT were significantly increased.[Conclusion]Poplar may respond to the invasion of F.solani(Mart.)Sacc by regulating sugar metabolism and transport,activating the ethyl-ene signaling pathway,promoting lignin accumulation,and thickening cell wall.

关 键 词：杨树 腐皮镰刀菌 侵染过程 转录组 

分 类 号：S763.15[农业科学—森林保护学]