文冠果种子发育基因表达分析内参基因的选择  

Selection of Reference Genes for Gene Expression Analysis in Seed Development of Xanthoceras sorbifolium Bunge

作　　者：李麟坤 梁重钧 宁楚龙 张薇 姜国维 张鑫[2] 王利兵[1,2] LI Lin-kun;LIANG Chong-jun;NING Chu-long;ZHANG Wei;JIANG Guo-Wei;ZHANG Xin;WANG Li-bing(Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Forestry College,Northwest A&F University,Yangling 712199,China)

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]西北农林科技大学林学院,陕西杨凌712199

出　　处：《林业科学研究》2024年第3期79-85,共7页Forest Research

基　　金：国家自然科学基金(31870594);西北农林科技大学高层次人才科研经费。

摘　　要：[目的]验证ACT基因是否适用于文冠果种子发育的相关基因表达分析,并筛选出在文冠果基因表达分析中最稳定的内参基因。[方法]本研究采用了4种数学算法(ΔCT、BestKeeper、NormFinder、geNorm)评估12个候选内参基因的表达稳定性,并利用在线工具RefFinder综合评价。[结果]在文冠果不同发育时期种子内参基因综合评分排名为PP2A>Tip41> EF-1α>18r>UCE>β-TUB>UBQ>α-TUB>CYP>SAM>ACT>GADPH;在文冠果不同组织内参基因综合评分排名为PP2A>Tip41>CYP>18r>UBQ>SAM>EF-1α>UCE>GADPH>α-TUB>ACT>β-TUB。[结论] PP2A和Tip41基因是文冠果基因表达分析最稳定的内参基因。[Objective]:To assess the suitability of the ACT gene for gene expression analysis in seed de-velopment of Xanthoceras sorbifolium,and to identify the most stable reference genes for gene expres-sion analysis in X.sorbifolium.[Method]:In this study,four mathematical algorithms(ΔCT,BestKeeper,NormFinder,geNorm)were carried out for evaluating the expression stability of 12 candidate reference genes based on the web-based tool RefFinder.[Result]:The RefFinder result showed that the gene PP2A performed the best,followed byTip41,EF-1α,18r,UCE,β-TUB,UBQ,α-TUB,CYP,SAM,ACT,and GADPH at the different seed development stages in X.sorbifolium,and the gene also had the highest score,followed by Tip41,CYP,18r,UBQ,SAM,EF-1α,UCE,GADPH,α-TUB,ACT,andβ-TUB at various tissues.[Conclusion]:PP2A and Tip41 are the two most stable reference genes for gene expression ana-lysis in X.sorbifolium.

关 键 词：文冠果 内参基因 基因表达 种子发育 

分 类 号：S727.32[农业科学—林木遗传育种]