连栽对木麻黄人工林根际土壤古菌群落变化的影响  

Effect of Continuous Planting on Archaeal Community Changes in Rhizosphere of Casuarina equisetifolia Plantations

作　　者：张清旭 李建鹃 王裕华 彭艳晖 王炎炎 郭玥 胡明玥 林文雄[1,3,4] 吴则焰 ZHANG Qing-xu;LI Jian-juan;WANG Yu-hua;PENG Yan-hui;WANG Yan-yan;GUO Yue;HU Ming-yue;LIN Wen-xiong;WU Ze-yan(College of JunCao Science and Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Forest Investigation and Planning Institute of Fujian Province,Fuzhou 350003,Fujian,China;Key Laboratory of Crop Ecology and Molecular Physiology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学菌草与生态学院,福建福州350002 [2]福建省林业调查规划院,福建福州350003 [3]福建农林大学作物生态与分子生理学福建省高校重点实验室,福建福州350002 [4]福建省农业生态过程与安全监控重点实验室,福建福州350002

出　　处：《林业科学研究》2024年第3期106-117,共12页Forest Research

基　　金：国家自然科学基金(31500443);福建省自然科学基金(2022J01139);福建省财政林业科技研究项目(2023FKJ26);福建农林大学科技创新专项(KFb22046XA、KJb22019XA)。

摘　　要：[目的]探究连栽对木麻黄人工林根际土壤古菌群落变化的影响,为探明木麻黄连栽障碍机制提供基础理论依据。[方法]以3个不同连栽代数(第一代First continuous plantation,FCP;第二代Second continuous plantation,SCP;第三代Third continuous plantation,TCP)的木麻黄人工林根际土壤为材料,以未种植木麻黄的天然乔木林根际土壤作为对照(CK),通过Illumina NovaSeq测序平台对古菌群落进行高通量测序。[结果]从3个不同连栽代数木麻黄人工林根际土壤和对照土壤中总共得到1 146 390条有效序列,以100%序列相似度聚类得到998 515个ASV。Alpha多样性分析结果表明,所观察到的物种数和Chao1指数均随着连栽代数的增加呈现下降的趋势,而Shannon指数、Simpson指数和pielou_e均以FCP最高,TCP次之,SCP最低。Beta多样性分析、PCoA分析、UPGMA聚类分析和Anosim分析结果表明,不同连栽代数木麻黄根际土壤古菌群落物种多样性存在差异,但不同组间差异不显著。共检测到5个门,优势菌门为奇古菌门(Thaumarchaeota),且奇古菌门占比随着栽植代数的增加呈现下降的趋势,与对照相比,FCP、SCP、TCP分别下降了17.42%、50.97%、51.51%。古菌群落与土壤理化性质相关性分析结果显示,奇古菌门与pH、全钾(TK)、全氮(TN)、全磷(TP)呈显著正相关,泉古菌门与速效磷(AP)和速效氮(AN)呈显著正相关,不同古菌群落与土壤理化因子之间的相关性存在显著差异。[结论]本研究证实了木麻黄人工林经过多代连续栽植后,根际土壤古菌群落结构发生显著变化,推测古菌群落结构失衡是导致土壤微生态失衡的重要原因之一。研究结果为揭示木麻黄人工林连栽障碍形成的原因提供了理论依据。[Objective]To investigate the impact of continuous planting on the changes in the archaeal com-munity in the rhizosphere soil of Casuarina equisetifolia Forst.plantations for understanding of the obstacle mechanism of continuous planting of C.equisetifolia and providing a solid theoretical basis for further ex-ploration.[Methods]We collected the rhizosphere soil from three C.equisetifolia plantations with varying generations of continuous planting(FCP,SCP,TCP).The rhizosphere soil from a natural arbor forest without C.equisetifolia was used as the control(CK).High-throughput sequencing of the archaeal com-munity was performed using the Illumina NovaSeq sequencing platform.[Results]A total of 1146390 ef-fective sequences were obtained from the rhizosphere soil samples and bare land control soil,resulting in the identification of 998515 ASVs with 100%sequence similarity.The analysis of Alpha diversity indicated that the observed number of species and Chao1 index decreased with successive generations.However,the Shannon index,Simpson index,and Pielou_e were highest in FCP,followed by TCP,and lowest in SCP.The results of the Beta diversity analysis,Principal Coordinate Analysis(PCoA),UPGMA cluster analysis,and Anosim analysis indicated variations in the species diversity of the archaeal community in the rhizosphere soil of C.equisetifolia across different continuous planting generations.However,the differ-ences between different groups were not significant.A total of 5 phyla were detected,and the dominant bacterial phylum was Thaumarchaeota and the proportion of Thaumarchaeota showed a decreasing trend with the increase of planting generations.Compared with the control,FCP,SCP and TCP decreased by 17.42%,50.97% and 51.51%respectively.The results of the correlation analysis between archaea com-munity and soil physical and chemical properties revealed significant positive correlations between Thau-marchaeota and pH,TK,TN and TP.Additionally,Crenarchaeota showed significant correlations with AP and AN.There were signifi

关 键 词：连栽障碍 木麻黄 高通量测序 根际 古菌群落 

分 类 号：S154.34[农业科学—土壤学]