基于SP/NK1R/βARRS通路电针缓解神经性疼痛的实验研究  

Experimental study of electroacupuncture mitigating neuralgia based on the SP/NK1R/βARRS signaling pathway

作　　者：王功命 刘世敏[1] 王凤娇 梁嘉仪 夏勇[1] 顾意鸣 王珂 具紫勇[1] WANG Gongming;LIU Shimin;WANG Fengjiao;LIANG Jiayi;XIA Yong;GU Yiming;WANG Ke;JU Ziyong(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《上海针灸杂志》2024年第6期684-690,共7页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81874506)。

摘　　要：目的观察针刺对坐骨神经慢性缩窄模型(chronic constriction injury,CCI)大鼠疼痛行为及脊髓背角P物质(substance P,SP)含量和神经激肽1受体(neurokinin 1 receptor,NK1R)、β-抑制蛋白1抗体(β-arrestin 1,βARR1)蛋白水平的影响。方法将36只雄性Sprague-Dawley(SD)大鼠随机分为假手术组、模型组、电针组和手针组,每组9只。暴露模型组、电针组和手针组大鼠左侧股骨中段坐骨神经并进行结扎,建立坐骨神经慢性缩窄模型;假手术组暴露坐骨神经但不结扎。造模后第8天开始,电针组和手针组分别对环跳和阳陵泉穴进行干预。测定4组大鼠造模后第0、7、13、21、29天的机械痛阈、热痛阈及后肢负重分布。采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测4组大鼠L4~L6腰膨大脊髓背角组织中SP含量;用蛋白质印迹法(Western blotting)检测4组大鼠L4~L6腰膨大脊髓背角组织中NK1R和βARR1蛋白表达。结果造模后第7天,模型组、电针组和手针组大鼠机械痛阈和热痛阈较假手术组降低(P<0.05),两后肢负重分布差异较假手术组增加(P<0.05)。针刺干预后,造模后第13、21、29天,手针组和电针组大鼠机械痛阈和热痛阈高于模型组(P<0.05),两后肢负重分布差异小于模型组(P<0.05);造模后第29天,电针组机械痛阈和热痛阈均高于手针组(P<0.05);造模后第21天,电针组两后肢负重分布差异小于手针组(P<0.05)。针刺干预全部结束后,模型组脊髓背角组织SP含量低于假手术组(P<0.05),手针组和电针组SP含量高于模型组(P<0.05)。针刺干预全部结束后,模型组脊髓背角组织NK1R和βARR1蛋白表达高于假手术组(P<0.05),电针组和手针组NK1R和βARR1蛋白表达低于模型组(P<0.05)。结论针刺可能通过调控脊髓背角SP水平及NK1R和βARR1蛋白表达对神经病理性疼痛起到镇痛效应。Objective To observe the effects of acupuncture on pain behaviors and the levels of substance P(SP)and proteins neurokinin 1 receptor(NK1R)andβ-arrestin 1(βARR1)in the spinal dorsal horn of rat models of chronic constriction injury(CCI)of the sciatic nerve.Method Thirty-six male Sprague-Dawley(SD)rats were randomized into a sham operation group,a model group,an electroacupuncture group,and a manual acupuncture group,with 9 rats in each group.In the model,electroacupuncture,and manual acupuncture groups,the sciatic nerve of rats was exposed and ligated by the middle of the left femur to establish the sciatic nerve CCI model.The sham operation group only received the exposure of the sciatic nerve but not ligation.From day 8 after modeling,the electroacupuncture and manual acupuncture groups received the corresponding intervention by selecting Huantiao(GB30)and Yanglingquan(GB34).On days 0,7,13,21,and 29 after modeling,the four groups of rats were assessed for their mechanical pain threshold,thermal pain threshold,and hind-limb weight distribution.The enzyme-linked immunosorbent assay(ELISA)was used to detect the content of SP in the L4-L6 spinal dorsal horn tissue of the four groups of rats;the protein expression of NK1R andβARR1 in the L4-L6 spinal dorsal horn tissue was determined by Western blotting.Result On day 7 after modeling,the mechanical and thermal pain thresholds were lower in the model,electroacupuncture,and manual acupuncture groups than in the sham operation group(P<0.05),and their hind-limb weight distribution difference was more significant compared with the sham operation group(P<0.05).After needling interventions,on days 13,21,and 29 after modeling,the mechanical and thermal pain thresholds were higher in the manual acupuncture and electroacupuncture groups than in the model group(P<0.05),and their hind-limb weight distribution difference was less significant than that in the model group(P<0.05);on day 29 after modeling,the electroacupuncture group had higher mechanical and thermal pain thresh

关 键 词：针刺疗法 电针 坐骨神经 神经痛 镇痛 脊髓背角 大鼠 

分 类 号：R2-03[医药卫生—中医学]