核桃青皮中萘醌类成分对银屑病小鼠抗氧化状态及炎症因子的影响  

Effects of naphthoquinone in walnut green husk on antioxidant status and inflammatory factors in psoriasis mice

作　　者：高洁 马婧怡 GAO Jie;MA Jingyi(Department of Pharmacy,the Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China;Office of Drug Clinical Trial Institutions,Zhuhai People's Hospital(Zhuhai Clinical Medical College of Jinan University),Zhuhai Guangdong 519000,China)

机构地区：[1]新疆医科大学第五附属医院药学部,乌鲁木齐830000 [2]珠海市人民医院(暨南大学珠海临床医学院)药物临床试验机构办公室,广东珠海519000

出　　处：《新疆医科大学学报》2024年第6期883-887,900,共6页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C442)。

摘　　要：目的研究核桃青皮中萘醌类成分对银屑病样炎症皮损小鼠抗氧化状态及炎症因子的影响。方法取60只Balb/c小鼠脱毛背部连续7 d涂抹5%咪喹莫特乳膏42 mg构建银屑病小鼠模型。将60只建模成功的小鼠随机分为5组,每组12只,分别为模型组(涂抹生理盐水10 mL)、阳性药对照组(涂抹卡泊三醇软膏42 mg)、核桃青皮中萘醌类成分高(涂抹60%流浸膏42 mg)、中(涂抹30%流浸膏42 mg)、低(涂抹15%流浸膏42 mg)剂量组。另取12只未建模小鼠为正常组(涂抹生理盐水10 mL)。连续干预10 d后,观察小鼠皮损变化并进行PASI评分;采用HE染色和Baker评分评估组织病理学损害程度;分光光度法检测银屑病小鼠背部皮肤组织中SOD、CAT、GSH-Px活性及MDA含量;采用ELISA法检测银屑病小鼠血清中TNF-α、IL-17和IL-23的含量。结果各组小鼠经相应药物干预10 d后,背部皮肤组织经HE染色镜下观察:正常组小鼠皮肤结构未见异常;模型组小鼠表皮层可见明显的Munro小脓肿,角化过度,颗粒层减少,棘层增厚明显,棘突延长起伏,真皮层可见散在轻微炎细胞浸润;阳性药对照组和核桃青皮萘醌类成分低、中、高剂量组小鼠皮肤组织病理改变和模型组类似,但表皮层角化过度、角化不全、颗粒层减少、棘层增厚等都较模型组有不同程度减轻。与正常组比较,模型组小鼠背部皮肤明显增厚,出现红斑和鳞屑,PASI评分升高,Baker评分升高,背部皮肤组织中T-SOD、CAT、GSH-Px的活性均降低,MDA的含量升高,血清中TNF-α、IL-17、IL-23含量均升高,差异有统计学意义(P<0.05)。与模型组比较,阳性药对照组和核桃青皮萘醌类成分低、中、高剂量组小鼠背部皮肤红肿程度减轻、鳞屑面积减少,PASI评分降低;阳性药对照组和核桃青皮萘醌类成分高剂量组T-SOD、CAT、GSH-Px的活性均升高,MDA的含量降低,Baker评分降低;阳性药对照组和核桃青皮中萘醌类成分中、高剂�Objective To study the effects of naphthoquinone in walnut green husk on antioxidant status and inflamma-tory factors in mice with psoriatic inflammatory lesions.Methods The psoriasis model of mice was induced by 42 mg 5%imquimod cream on the back of 60 Balb/c mice for 7 consecutive days.60 successfully modeled mice were ran-domly divided into 5 groups,with 12 mice in each group,namely the model group(coated with 10 mL of physiological saline),the positive drug control group(coated with 42 mg of carbopol ointment),the high-dose group of naphtho-quinone compounds in walnut green skin(coated with 42 mg of 60%fluid extract),the medium dose group(coated with 42 mg of 30%fluid extract),and the low-dose group(coated with 42 mg of 15%fluid extract).Another 12 un-modeled mice were selected as normal group(coated with 10 mL of physiological saline).After 10 consecutive days of intervention,the changes of skin lesions were observed and PASI score was performed;the skin lesions were taken by HE staining,and the histopathological lesion was evaluated using Baker score;the activity of SOD,CAT,GSH-Px and the content of MDA in the back skin tissue of psoriasis mice were detected by spectrophotometry;the contents of TNF-α,IL-17 and IL-23 in serum of psoriatic mice were detected by ELISA.Results After 10 days of intervention with corresponding drugs,the back skin tissue of each group of mice was observed under HE staining microscopy:no abnormalities were found in the skin structure of the normal group of mice;The model group mice showed obvious Munro small abscesses in the epidermal layer,excessive keratinization,reduced granular layer,significantly thickened spinous layer,elongated and undulating spinous processes,and scattered mild inflammatory cell infiltration in the der-mis;The pathological changes in the skin tissue of mice in the positive drug control group and the low,medium,and high dose groups of walnut green skin naphthoquinones were similar to those in the model group,but the excessive and incomplete keratinization o

关 键 词：核桃青皮 萘醌 银屑病 抗氧化 炎症因子 

分 类 号：R965[医药卫生—药理学]