Porous nanofibrous dressing enables mesenchymal stem cell spheroid formation and delivery to promote diabetic wound healing  

作　　者：Kexin Zhang Wenmin Zhang Heng An Zhe Huang Yanzhen Wen Xiangyu Jiao Yongqiang Wen 

机构地区：[1]College of Chemical Engineering and Technology,Taiyuan University of Science and Technology,Taiyuan 030024,China [2]Beijing Key Laboratory for Bioengineering and Sensing Technology,Daxing Research Institute,School of Chemistry&Biological Engineering,University of Science&Technology Beijing,Beijing 100083,China

出　　处：《Chinese Journal of Chemical Engineering》2024年第4期156-164,共9页中国化学工程学报（英文版）

基　　金：supported by Fundamental Research Program of Shanxi Province(202203021222199);the Taiyuan University of Science and Technology Scientific Research Initial Funding(20222090);the National Natural Science Foundation of China(21975019).

摘　　要：Delayed and nonhealing of diabetic wounds imposes substantial economic burdens and physical pain on patients.Mesenchymal stem cells(MSCs)promote diabetic wound healing.Particularly when MSCs aggregate into multicellular spheroids,their therapeutic effect is enhanced.However,traditional culture platforms are inadequate for the efficient preparation and delivery of MSC spheroids,resulting in inefficiencies and inconveniences in MSC spheroid therapy.In this study,a three-dimensional porous nanofibrous dressing(NFD)is prepared using a combination of electrospinning and homogeneous freeze-drying.Using thermal crosslinking,the NFD not only achieves satisfactory elasticity but also maintains notable cytocompatibility.Through the design of its structure and chemical composition,the NFD allows MSCs to spontaneously form MSC spheroids with controllable sizes,serving as MSC spheroid delivery systems for diabetic wound sites.Most importantly,MSC spheroids cultured on the NFD exhibit improved secretion of vascular endothelial growth factor,basic fibroblast growth factor,and hepatocyte growth factor,thereby accelerating diabetic wound healing.The NFD provides a competitive strategy for MSC spheroid formation and delivery to promote diabetic wound healing.

关 键 词：Electrospinning Homogenization Biomedical engineering Nanomaterials Stem cell spheroids Diabetic wound healing 

分 类 号：R587.1[医药卫生—内分泌] TB383.1[医药卫生—内科学]