Biocatalytic enhancement of laccase immobilized on ZnFe_(2)O_(4) nanoparticles and its application for degradation of textile dyes  

作　　者：Yuhang Wei Qingpeng Zhu Weiwei Xie Xinyue Wang Song Li Zhiming Chen 

机构地区：[1]School of Chemical and Environmental Engineering,Anhui Laboratory of Functional Coordinated Complexes for Materials Chemistry and Application,Anhui Polytechnic University,Wuhu 241000,China [2]School of Biology and Food Engineering,Anhui Polytechnic University,Wuhu 241000,China

出　　处：《Chinese Journal of Chemical Engineering》2024年第4期216-223,共8页中国化学工程学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(21471002);Scientific Research Projects of Universities in Anhui Province(2022AH040135);Natural Science Research Project for Anhui Universities(KJ2021A0509);Anhui Natural Science Foundation(2208085MC83).

摘　　要：Efficient and convenient treatment of industrial dyeing wastewater is of great significance to guarantee human and animal health.This work presented the enhanced catalytic activity at pH 3.0 of laccase immobilized on amino-functionalized ZnFe_(2)O_(4) nanoparticles(ZnFe_(2)O_(4)-laccase)and its application for the degradation of textile dyes.Due to the existence of a large number of oxygen vacancies on the surface of the ZnFe_(2)O_(4) nanoparticles,negative ions accumulated on the magnetic carriers,which resulted in a harsh optimal pH value of the ZnFe_(2)O_(4)-laccase.Laccase activity assays revealed that the ZnFe_(2)O_(4)-laccase possessed superior pH and thermal stabilities,excellent reusability,and noticeable organic solvent tolerance.Meanwhile,the ZnFe_(2)O_(4) laccase presented efficient and sustainable degradation of high concentrations of textile dyes.The initial decoloration efficiencies of malachite green(MG),brilliant green(BG),azophloxine,crystal violet(CV),reactive blue 19(RB19),and procion red MX-5B were approximately 99.1%,95.0%,93.3%,87.4%,86.1%,and 85.3%,respectively.After 10 consecutive reuses,the degradation rates of the textile dyes still maintained about 98.2%,92.5%,83.2%,81.5%,79.8%and 65.9%,respectively.The excellent dye degradation properties indicate that the ZnFe_(2)O_(4)-laccase has a technical application in high concentrations of dyestuff treatment.

关 键 词：ZnFe_(2)O_(4)-laccase Catalytic activity Stability and reusability Degradation of textile dye 

分 类 号：TQ426.97[化学工程] X791[环境科学与工程—环境工程]