Exploring the mechanism of active ingredients of Smilacis Glabrae Rhizoma in treating migraine headache through network pharmacology and animal experiments  

作　　者：Dao-Xian Shu Yan-Yan Chen Jiao-Rong Wang Ying Wang Yong-Fang Gu Yan-Xin Wang 

机构地区：[1]Department of Brain DiseaseⅡ,The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230031,China

出　　处：《Integrative Medicine Discovery》2024年第18期1-10,共10页

基　　金：supported by Open Fund Project of Key Laboratory of Xin’an Medicine Ministry of Education(2020xayx09);Scientific Research Program of Chinese Society of Ethnomedicine(2020Z1066-410113).

摘　　要：Background:To explore the potential mechanism of action of the active ingredients of Smilacis Glabrae Rhizoma(SGR)in the treatment of migraine using network pharmacology and in vivo experiments.Methods:Through the search of Traditional Chinese Medicines Systems Pharmacology Database and Analysis Platform,Genecards,Drugbank and other databases,we obtained active ingredients,targets of SGR and related disease targets of migraine,and took the intersection for protein-protein interactions analysis.After constructing the network diagram,network topology analysis was performed to derive the core targets and key active ingredients,and Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed.Finally,molecular docking was performed and validated by in vivo experiments.In vivo experiments,18 male BALB/c mice were selected,and the SGR group was fed with SGR drinking tablet concentrate,and nitroglycerin injection was used to construct a mouse model of migraine.Enzyme-linked immunosorbent assay test was used to detect the levels of TNF-α,IL-1β,IL-6,and AKT1 in plasma.Results:The results showed that the core targets of SGR for the treatment of migraine were TNF-α,IL-1β,IL-6,and AKT1.These core targets and key active ingredients had better binding ability.Compared with the blank group,the number of head scratching in the model group increased.Compared with the model group,there was a significant reduction of the number of head scratching in the SGR group.In comparison with the blank group,the protein level in the plasma in the model group was markedly higher.Compared with the model group,the protein level in the SGR group was significantly lower.Conclusion:SGR has the characteristics of improving migraine based on multi-targets,multi-components and multi-pathways,and the mechanism of action may be related to the inhibition of the release of inflammatory factors,neuron protection,and interference with apoptosis and other processes.

关 键 词：MIGRAINE Smilacis Glabrae Rhizoma network pharmacology molecular docking mechanism of effect 

分 类 号：R285[医药卫生—中药学]