战骨叶甲醇部位化学成分及其抗炎活性研究  

Chemical constituents from the methanol fraction of Premna fulva leaves and their anti-inflammatory activities

作　　者：贺非凡 赵宁涛 颜小捷[1] 卢凤来[1] 蒋小华[1] 李和平[2] 李典鹏[1] 陈月圆[1] HE Fei-fan;ZHAO Ning-tao;YAN Xiao-jie;LU Feng-lai;JIANG Xiao-hua;LI He-ping;LI Dian-peng;CHEN Yue-yuan(Guangxi Institute of Botany,Chinese Academy of Science,Guangxi Zhuang Autonomous Region/Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization,Guilin 541006,China;Guilin University of Technology,Guilin 541006,China)

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西植物功能物质与资源持续利用重点实验室,广西桂林541006 [2]桂林理工大学,广西桂林541006

出　　处：《中成药》2024年第6期1894-1899,共6页Chinese Traditional Patent Medicine

基　　金：广东省重点区域研究发展规划(2020B1111110003);广西自然科学基金项目(2020GXNSFBA297055);桂林市创新平台和人才计划(20210102-3)。

摘　　要：目的研究战骨叶甲醇部位的化学成分及其抗炎活性。方法战骨叶甲醇部位采用薄层色谱、柱色谱和HSCCC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用RAW264.7模型评价其体外抗炎活性。结果从中分离得到12个化合物,分别为鉴定为牡荆素(1)、balanophonin(2)、桦褐孔菌二糖(3)、4-allyl-2,6-dimethoxyphenol-β-D-glucopyranoside(4)、去氢催吐萝芙木醇(5)、地芰普内酯(6)、(E)-4-((1S,3R,4R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl)but-1-en-3-o-ne(7)、(E)-4-hydroxyphenylprop-7-ene 4-O-β-D-glucopyranoside(8)、4-羟基苯甲酸4-O-β-D-吡喃葡萄糖苷(9)、vicenin(10)、齐墩果酸(11)、芝麻素(12)。化合物1、2、5、7、10、12对NO均具有良好的抑制活性,IC 50值分别为(26.42±2.5)、(21.24±2.2)、(25.88±1.9)、(29.72±2.1)、(8.90±1.1)、(9.73±0.7)μmol/L。结论化合物2、4~8为首次从豆腐柴属植物中分离得到。化合物1、2、5、7、10、12具有抗炎活性。AIM To study the chemical constituents from the methanol fraction of Premna fulva Craib leaves and their anti-inflammatory activities.METHODS The methanol fraction from P.fluva were isolated and purified by TLC,column chromatography,and HSCCC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities in vitro were evaluated by RAW264.7 model.RESULTS Twelve compounds were isolated and identified as vitexin(1),balanophonin(2),inotodisaccharide(3),4-allyl-2,6-dimethoxyphenol-β-D-glucopyranoside(4),dehydrovomifoliol(5),loliolide(6),(E)-4-((1S,3R,4R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl)but-1-en-3-o-ne(7),(E)-4-hydroxyphenylprop-7-ene 4-O-β-D-glucopyranoside(8),4-O-β-D-glucopyranosylbenzoic acid(9),vicenin(10),oleanicacid(11),sesamin(12),respectively.Compounds 1,2,5,7,10,and 12 showed good inhibitory activities against NO,and the IC 50 values were(26.42±2.5)、(21.24±2.2)、(25.88±1.9)、(29.72±2.1)、(8.90±1.1)、(9.73±0.7)μmol/L,respectively.CONCLUSION Compounds 2,4-8 are isolated from Premna genus plants for the first time.Compounds 1,2,5,7,10,12 have anti-inflammatory activities.

关 键 词：战骨叶 甲醇部位 化学成分 分离鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]