基于金纳米颗粒的球形核酸用于脑肿瘤基因治疗  

Spherical Nucleic Acid Based on Gold Nanoparticles for Gene Therapy of Brain Tumor

作　　者：程和美 CHENG He-mei(College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学化学化工学院,湖北武汉430062

出　　处：《安徽化工》2024年第3期88-91,95,共5页Anhui Chemical Industry

摘　　要：基因治疗作为脑肿瘤常用的治疗方法,因反义寡核苷酸难以被细胞摄取,限制其治疗效果。为了提高反义寡核苷酸(DNA)的治疗效果,设计了一种金纳米颗粒,在其表面结合大量DNA,制备球形核酸(Au-DNA),用于脑肿瘤基因治疗。Au-DNA为核壳结构,粒径100 nm,电势为负。由于Au-DNA特殊的三维纳米结构,更容易被肿瘤细胞摄取,其摄取效率是游离DNA的4倍,可将更多的DNA递送至细胞内,并且细胞对Au-DNA的摄取是时间依赖的。由于Au-DNA较高的细胞摄取效率,可降低生存素蛋白表达水平,能有效抑制脑肿瘤细胞生长。Gene therapy is commonly used to treat brain tumors,but antisense oligonucleotides are difficult to be taken up by cells,which limits the therapeutic effect.In order to improve the therapeutic effect of antisense oligonucleotide(DNA),spherical nucleic acid(Au-DNA)was prepared by binding a large amount of DNA on the surface of gold nanoparticle for tumor gene therapy.Au-DNA was a core-shell structure with a particle size of 100 nm and a negative potential.Due to its special three-dimensional nanostructure,Au-DNA was easier to be taken up by tumor cells.And the cellular uptake efficiency of Au-DNA was about four times as high as that of free DNA,which can deliver more DNA into cells.And cell uptake of Au-DNA was time dependent.Due to the high cell uptake efficiency of Au-DNA,the expression level of survivin protein can be reduced,which can effectively inhibit the growth of brain tumor cells.

关 键 词：球形核酸 基因治疗 脑肿瘤 生存素蛋白 

分 类 号：O657[理学—分析化学]