人绒毛膜促性腺激素辅助治疗对隐睾大鼠生殖功能的影响  

Effect of adjunct human chorionic gonadotropin therapy on reproductive function in cryptorchid rats

作　　者：唐浩茴 郝春生[2] Tang Haohui;Hao Chunsheng(Capital Institute of Pediatrics,Beijing 100020,China;Department of Pediatric ULrology,Affiliated Children's Hospital,Capital Institute of Pediatrics,Beijing 100020,China)

机构地区：[1]首都儿科研究所,北京100020 [2]首都儿科研究所附属儿童医院泌尿外科,北京100020

出　　处：《中华小儿外科杂志》2024年第5期441-448,共8页Chinese Journal of Pediatric Surgery

基　　金：北京市医院管理局重点医学专业发展计划(ZYLX201825)。

摘　　要：目的探究人绒毛膜促性腺激素辅助治疗对单侧隐睾大鼠行睾丸固定术后患、健侧睾丸生殖功能的影响。方法使用氟他胺处理实验组孕鼠,诱导其子代雄鼠产生隐睾表型;同时使用玉米油处理对照组孕鼠。数字随机法选取15只21 d龄单侧隐睾雄鼠,分为隐睾组、手术组和手术药物组;选取5只21 d龄健康雄鼠作为对照组。对照组和隐睾组行单侧假手术操作,手术组和手术药物组行单侧睾丸固定术。术后第3天和第10天,对照组、隐睾组和手术组皮下注射1 mL生理盐水,手术药物组皮下注射50 U(1 mL)人绒毛膜促性腺激素。于大鼠60 d龄时取右心房血液、双侧睾丸、附睾标本,双侧睾丸称重记录后分别计算睾丸/体重脏器系数;检测健、患侧附睾精子数量;检测双侧睾丸组织平均曲细精管直径和平均生精上皮细胞厚度;ELISA检测血清卵泡刺激素、促黄体生成素和睾酮浓度;TUNEL法检测生殖细胞凋亡率。结果手术药物组患侧睾丸重量较手术组明显增高[(0.63±0.13)g比(0.37±0.06)g,P<0.01],睾丸/体重脏器系数较手术组明显增高[(2.00±0.39)‰比(1.18±0.17)‰,P<0.001];手术药物组患侧睾丸曲细精管直径显著大于手术组[(152.1±11.5)μm比(137.2±16.2)μm,P<0.001],生精细胞厚度显著大于手术组[(40.1±8.4)μm比(32.9±6.6)μm,P<0.001];隐睾组、手术组和手术药物组患侧睾丸生殖细胞凋亡率差异无统计学意义(P>0.05)。手术组、手术药物组血清卵泡刺激素、促黄体生成素和睾酮浓度差异无统计学意义(P>0.05)。四组大鼠的健侧睾丸重量、睾丸/体重脏器系数、曲细精管直径、生精细胞厚度、生殖细胞凋亡率、附睾精子数量差异均无统计学意义(P>0.05)。结论人绒毛膜促性腺激素辅助治疗在一定程度上可以改善氟他胺诱导的单侧隐睾大鼠行睾丸固定术后患侧睾丸的生殖功能,对健侧睾丸生殖功能影响较小。ObjectiveTo explore the effect of adjunct human chorionic gonadotropin(hCG)therapy on bilateral testicular reproductive function after orchiopexy in unilateral cryptorchidism rats and to provide animal experimental rationales for adjunct hormone therapy of children with cryptorchidism.MethodsThe pregnant rats in experimental group recieved flutamide for inducing their offspring to develop cryptorchidism phenotype while the pregnant rats in control group were treated with corn oil.Fifteen 21-day-old unilateral cryptorchidism rats were randomized into 3 groups of cryptorchidism,orchiopexy and orchiopexy plus hCG.And five 21-day-old healthy male rats were selected as control group.Ochiopexy was performed in orchiopexy and orchiopexy plus hCG groups.Sham operation was performed in control and cryptorchidism groups.At Day 3/10 post-operation,50 U(1 ml)hCG was hypodermically injected in rats of orchiopexy plus hCG group.And 1 mL normal saline was hypodermically injected in three other groups.At Day 60 post-birth,the rats were enthanized and samples of blood,testes and epididymides collected.Testicular weight,testicular organ coefficent and sperm count were measured.Mean diameter of seminefrous tubule and thickness of germinal epithelial cell were calculated.The concentrations of follicle stimulating hormone(FSH),luteinizing hormone(LH)and testosterone(T)were measured by enzyme-linked immunosorbent assay(ELISA).The levels of germ cell apoptosis were examined with TUNEL method and apoptotic rate was calculated.ResultsThe weight of affected testis was significantly higher in orchiopexy plus hCG group than that in orchiopexy group[(0.63±0.13)vs(0.37±0.06)g,P<0.01].And testicular organ coefficent of affected testis showed the same result[(2.00±0.39)‰vs(1.18±0.17)‰,P<0.001].Mean diameter of seminefrous tubule was higher in orchiopexy plus hCG group than that in orchiopexy group[(152.1±11.5)vs(137.2±16.2)μm,P<0.001].And the same result was found for mean thickness of germinal epithelial cell[(40.1±8.4)vs(32.9±6.6

关 键 词：隐睾 人绒毛膜促性腺激素 生殖功能 氟他胺 大鼠 

分 类 号：R726.9[医药卫生—儿科]