桂枝加龙骨牡蛎汤对肺不藏魄型不寐模型大鼠IL-10及TNF-α表达的影响  

Influence of Guizhi Jia Longgu Mulitang on Expression of IL-10 and TNF-αin Insomnia Rats with Sensory Dysfunction Dominated by Lung

作　　者：吴金鸿 张星平 闫德祺 梁瑞宁 陈旭 梁政亭 贾宏林 王慧 WU Jinhong;ZHANG Xingping;YAN Deqi;LIANG Ruining;CHEN Xu;LIANG Zhengting;JIA Honglin;WANG Hui(The Fourth Clinical College of Xinjiang Medical University,Xinjiang Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830099,China)

机构地区：[1]新疆医科大学第四临床医学院,新疆维吾尔自治区中医医院,乌鲁木齐830099

出　　处：《中国实验方剂学杂志》2024年第13期20-27,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(82160873,81960837,81560762,81260526);新疆维吾尔自治区自然科学基金项目(2022D01D48);国家中医药管理局“十四五”高水平中医药重点学科项目(国中医药人教函[2023]85号);全国名老中医药专家传承工作室项目(国中医药人教函[2022]75号)。

摘　　要：目的:探讨桂枝加龙骨牡蛎汤对肺不藏魄型不寐大鼠相关脏器中白细胞介素(IL)-10及肿瘤坏死因子(TNF)-α表达差异的影响,研究桂枝加龙骨牡蛎汤改善不寐的作用机制。方法:将SD大鼠随机分为空白组、模型组、西药组、中药组,每组10只。造模方法选用长平台浅水环境法剥夺大鼠睡眠,造模共计42 d。空白组和模型组给予0.05 mL·kg^(-1)生理盐水灌胃,将西药组、中药组大鼠在继续造模的同时给予干预,西药组给予右佐匹克隆片0.105 mg·kg^(-1)灌胃,中药组给予桂枝加龙骨牡蛎汤7600 mg·kg^(-1)灌胃,共干预28 d。造模成功后,第42天开始进行Morris水迷宫实验检测大鼠的运动及空间记忆能力;以酶联免疫吸附测定法(ELISA)检测大鼠血清中IL-10及TNF-α的水平;蛋白免疫印迹法(Western blot)检测大鼠肺组织和脑组织的IL-10及TNF-α蛋白表达;免疫组化法检测大鼠肺组织和脑组织的IL-10及TNF-α的水平。结果:与空白组比较,模型组大鼠睡眠分期非快速眼球运动(NREM)期和快速眼球运动(REM)期时间明显缩短(P<0.05,P<0.01),觉醒期时间显著增加(P<0.01),平台探索总时间及总距离显著增加(P<0.01),目标象限(第三象限)探索时间百分比及穿越平台次数显著降低(P<0.01),ELISA结果显示血清中IL-10水平显著降低(P<0.01),TNF-α水平显著升高(P<0.01),Western blot结果显示大鼠脑、肺组织IL-10蛋白表达显著降低(P<0.01),TNF-α蛋白表达显著升高(P<0.01),免疫组化法检测结果显示大鼠脑、肺组织IL-10表达显著降低(P<0.01),TNF-α表达显著升高(P<0.01)。与模型组比较,西药组及中药组大鼠睡眠分期NREM期和REM期时间明显增加(P<0.05,P<0.01),觉醒期时间缩短(P<0.05),平台探索总时间及总距离显著降低(P<0.01),目标象限(第三象限)探索时间百分比及穿越平台次数明显增加(P<0.05,P<0.01),ELISA结果显示血清中IL-10水平显著升高(P<0.01),血清中TNF-α水平显著降低(P<0Objective:To investigate the effects of Guizhi Jia Longgu Mulitang on the expression difference of interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)in related organs of insomnia rats with sensory dysfunction dominated by lung and study the mechanism of Guizhi Jia Longgu Mulitang in improving insomnia.Method:SD rats were randomly divided into the blank group,model group,western medicine group,and traditional Chinese medicine(TCM)group,with 10 rats in each group.The rats were deprived of sleep by shallow water environment method in a long platform,and the modeling lasted for 42 d.The blank group and model group were given 0.05 mL·kg^(-1)normal saline by gavage,and the western medicine group and TCM group were given drugs during modeling.To be specific,the western medicine group was given 0.105 mg·kg^(−1) dexzopiclone tablet by gavage,while the TCM group was given 7600 mg·kg^(-1)Guizhi Jia Longgu Mulitang by gavage,both lasting for 28 days.After successful modeling,the Morris water maze experiment was performed on the 42nd day to detect the motion and spatial memory ability of rats.The levels of IL-10 and TNF-αin serum were detected by enzyme-linked immunosorbent assay(ELISA).The protein expression of IL-10 and TNF-αin the lung and brain tissue of rats was detected by Western blot.The levels of IL-10 and TNF-αin the lung and brain tissue of rats were detected by immunohistochemistry.Result:Compared with the blank group,the sleep stages non-rapid eye movement(NREM)and rapid eye movement(REM)of the model group were significantly shortened(P<0.5,P<0.01),and the wake stage was significantly increased(P<0.01).The total time and distance of platform exploration were significantly increased(P<0.01).In the target quadrant(the third quadrant),the percentage of exploration time and the times of crossing the platform were significantly decreased(P<0.01).ELISA results showed that the serum IL-10 level was significantly decreased(P<0.01),and TNF-αlevel was significantly increased(P<0.01).The results of Western b

关 键 词：桂枝加龙骨牡蛎汤 肺不藏魄 不寐 炎症因子 中医不寐五神分型诊断法 

分 类 号：R2-0[医药卫生—中医学] R22R242R285.5