沙棘黄酮对长期力竭运动大鼠心肌保护作用的机制研究  

Mechanism of sea-buckthorn flavone on myocardial protection in rats after long-term exhausted exercise

作　　者：孔海军 谌晓安[2] 李新龙 KONG Hai-jun;CHEN Xiao-an;LI Xin-long(College of Physical Education,Kashi University,Kashi 844000,China;College of Physical Education Science,Jishou Uviversity,Xiangxi 416000,China)

机构地区：[1]喀什大学体育学院,喀什844000 [2]吉首大学体育科学学院,湘西416000

出　　处：《天然产物研究与开发》2024年第6期954-962,共9页Natural Product Research and Development

基　　金：新疆维吾尔自治区自然科学基金(Y2021S037);新疆维吾尔自治区高校科技计划(XJEDU2021SY040);喀什地区科技计划(KS2020024)。

摘　　要：观察沙棘黄酮(sea-buckthorn flavone,SF)对长期力竭运动大鼠心肌组织的保护作用。50只6周龄SPF级雄性SD大鼠随机分为正常组、模型组和SF低中高剂量组(100、200、400 mg/kg)。通过连续6周力竭跑台训练建立运动性心肌损伤模型。ELISA法检测心肌炎症反应、氧化应激相关指标及血清心肌损伤标志物;免疫组织化学法检测心肌半胱天冬酶3(cysteinyl aspartate specific proteinase-3,Caspase-3)蛋白表达;免疫印迹法检测心肌Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、B淋巴细胞瘤-2基因(B cell iymphoma-2,Bcl-2)、Ⅰ型胶原(type I Collagen,Collagen-Ⅰ)、结缔组织生长因子(connective tissue growth factor,CTGF)蛋白表达;HE、Masson染色观察心肌组织形态及胶原沉积。结果表明,与模型组比较,SF各剂量组大鼠血清谷草转氨酶(aspartate aminotransferase,AST)、肌酸激酶同工酶MB(creatine kinase MB,CK-MB)、心肌肌钙蛋白Ⅰ(cardiac troponinⅠ,cTnⅠ)、心肌组织胶原容积(collagen volume fraction,CVF)水平和心肌组织Caspase-3、Bax、Collagen-Ⅰ蛋白表达显著降低(P<0.01),心肌组织Bcl-2蛋白表达和Bcl-2/Bax比值显著上升(P<0.01);中高剂量SF还可显著降低大鼠心肌组织丙二醛(malondialdehyde,MDA)、白介素1-β(interleukin-1β,IL-1β)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平和心肌组织CTGF蛋白表达(P<0.01),提高心肌组织超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平(P<0.01)。研究结果提示,中、高剂量SF可抑制长期力竭训练大鼠心肌组织氧化应激、炎症反应、细胞凋亡和纤维化,减轻心肌损伤,且高剂量作用效果更佳。The study was conducted to observe the protective effect of sea-buckthorn flavone(SF)on myocardium in rat subjected to long-term exhausted exercise.Fifty six-week-old SPF-grade male SD rat were randomly divided into normal group,model group,and SF low,medium and high dose(100,200 and 400 mg/kg)group.An exercise induced myocardial injury model was established through six-week exhausted treadmill training.Inflammatory reaction in myocardium,oxidative stress related index and the serum markers of myocardial injury were detected by ELISA;myocardial cysteinyl aspartate specific proteinase-3(Caspase-3)protein expression was detected by the immunohistochemical method;protein expressions of myocardial BCL2-associated X(Bax),B-cell lymphoma-2(Bcl-2),type I Collagen(Collagen-Ⅰ),and connective tissue growth factor(CTGF)were detected by Western blot;myocardial tissue morphology and collagen deposition were observed by HE and Masson staining.The results showed that compared with the model group,the levels of serum aspartate aminotransferase(AST),creatine kinase MB(CK-MB),cardiac troponin I(cTn I),collagen volume fraction(CVF),and the expression of Caspase-3,Bax,and Collagen-I proteins in the myocardial tissue of rats in each dose group of SF were significantly reduced(P<0.01),while the expression of Bcl-2 protein and Bcl-2/Bax ratio in the myocardial tissue were significantly increased(P<0.01);Medium to high doses of SF can significantly reduce malondialdehyde(MDA),interleukin-1β(IL-1β),Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and expression of CTGF protein in myocardial tissue(P<0.01),increase the levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in myocardial tissue(P<0.01).The results of the study suggested that medium and high doses of SF could inhibit oxidative stress,inflammatory reaction,cell apoptosis,and fibrosis in the myocardial tissues of rat subjected to long-term exhausted exercise,and alleviate myocardial injury.It was found that the effect of a high dose was better.

关 键 词：力竭运动 沙棘黄酮 心肌损伤 细胞凋亡 纤维化 

分 类 号：R873[医药卫生—运动医学]