野菊中一个新的吉马烷型倍半萜  

A new gemmarane-type sesquiterpenoid from Chrysanthemum indicum L.

作　　者：潘浩 薛金凤 贾瑶瑶 卢纪衡 袁雨洁 冯卫生 吴亚 薛贵民 PAN Hao;XUE Jin-feng;JIA Yao-yao;LU Ji-heng;YUAN Yu-jie;FENG Wei-sheng;WU Ya;XUE Gui-min(The Engineering and Technology Center for Chinese Medicine Development of Henan Province,School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南省中药开发工程技术研究中心,郑州450046

出　　处：《天然产物研究与开发》2024年第6期963-967,共5页Natural Product Research and Development

基　　金：国家自然科学基金(82003606);河南省高校科技创新团队支持计划(24IRTSTHN039)。

摘　　要：研究野菊Chrysanthemum indicum L.地上部分中萜类成分及其抗炎活性。采用MCI、ODS、SephadexLH-20凝胶柱色谱和pre-HPLC等方法分离纯化,再通过对其波谱数据分析鉴定化合物的结构。从野菊二氯甲烷提取物中分离纯化得到6个单体化合物,分别是chrygermalide A(1)、3β-hydroxy-oxo-7α,11β-germacra-4 Z,10(14)-diene-12,6α-olide(2)、deacetylherbolide A(3)、9β-hydroxy costunolide(4)、3β-hydroxycostunolide(5)、3β-hydroxy-11α,13-dihydrocos-tunolide(6)。其中化合物1为新化合物,2~6为首次从该属植物中分离得到。采用LPS诱导RAW 264.7巨噬细胞的炎症模型测试了化合物1~6的抗炎活性,其NO抑制率IC 50值在17.9~33.9μmol/L之间。To study the chemical constituents of the aboveground of Chrysanthemum indicum L.All compounds were separated and purified by MCI,ODS,SephadexLH-20 gel column chromatography and pre-HPLC,and the structures of them were identified by analyzing of their spectroscopic data.Compounds 1-6 were isolated from the dichloromethane extract of C.indicum,which were identified as chrygermalide A(1),3β-hydroxy-oxo-7α,11β-germacra-4 Z,10(14)-diene-12,6α-olide(2),deacetylherbolide A(3),9β-hydroxy costunolide(4),3β-hydroxycostunolide(5),3β-hydroxy-11α,13-dihydrocos-tunolide(6).Compound 1 is a new compound,and 2-6 are isolated from this genus for the first time.The anti-inflammatory activity of compounds 1-6 were tested in LPS-induced RAW 264.7 macrophage cells,and the IC 50 values of their NO release inhibition were 17.9-33.9μmol/L.

关 键 词：野菊 化学成分 倍半萜类 抗炎活性 

分 类 号：R284.2[医药卫生—中药学]