补骨脂醇提物UPLC-Q-TOF-MS化学成分分析及促黑色素生成作用机制初探  

Chemical composition analysis of Psoraleae Fructus alcohol extract by UPLC-Q-TOF-MS and its mechanisms of promoting melanogenesis

作　　者：何婷 王银洁[2] 詹莹莹 莫婷 杨丽娟 杨森 HE Ting;WANG Yin-jie;ZHAN Ying-ying;MO Ting;YANG Li-juan;YANG Sen(Ningxia Hui Medicine Research Institute,Yinchuan 750021,China;Yinchuan Hospital of Traditional Chinese Medicine Affiliated to the Ningxia Medical University,Yinchuan 750001,China;The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]宁夏回族医药研究所,银川750021 [2]宁夏医科大学附属银川市中医医院,银川750001 [3]广州中医药大学第二临床医学院,广州510006

出　　处：《天然产物研究与开发》2024年第6期968-981,953,共15页Natural Product Research and Development

基　　金：宁夏自然科学基金(2023AAC03717);宁夏青年科技人才托举工程项目(2020-78);宁夏回族自治区卫生健康委员会2023年度科研项目(2023-NWKYP-038)。

摘　　要：分析补骨脂醇提物(Psoraleae Fructus alcohol extract,PFE)化学成分并探讨其促黑色素生成作用机制。采用超高效液相色谱-四级杆飞行时间质谱(UPLC-Q-TOF-MS)对PFE化学成分进行鉴定;观察PFE对斑马鱼黑色素生成的影响;通过实时荧光定量聚合酶链式反应(quantitative real-time PCR,qRT-PCR)考察PFE、补骨脂素和异补骨脂素对小鼠黑色素瘤细胞B16-F10中酪氨酸酶(tyrosinase,Tyr)、酪氨酸酶相关蛋白1(Tyr-related protein 1,Tyrp1)和相关蛋白2(Tyr-related protein 2,Tyrp2)mRNA表达的影响。结果在PFE中共鉴定47个化学成分,其中黄酮类27个,香豆素类10个,单萜酚类8个和其他类2个;发现PFE作用斑马鱼18 hpf(受精后小时,hours post fertilization)后,能明显增加其体内黑色素水平;qRT-PCR结果显示,与对照组相比,PFE能显著增加Tyr和Tyrp2 mRNA的表达,其他给药组均能不同程度地增加Tyr、Tyrp1和Tyrp2 mRNA的表达。This study aims to analyze the chemical constituents of Psoraleae Fructus ethanol extract(PFE)and explore its mechanism of promoting melanin production.The chemical constituents of PFE were analyzed and identified by ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS)technology.Zebrafish was used to investigate the effects of PEF on the melanogenesis.The regulation effects of PFE,psoralen and isopsoralen on the mRNA expression of tyrosinase(Tyr),Tyr-related protein 1(Tyrp1)and Tyr-related protein 2(Tyrp2)in mouse melanoma cells(B16-F10)were detected by real-time fluorescence quantitative PCR(qRT-PCR).A total of 47 compounds were identified in PFE extract,including 27 flavonoids,ten coumarins,eight monoterpenoids and two other compounds.Meanwhile,the Zebrafish experiment results showed that PFE could significantly increase the melanin level in zebrafish after 18 hpf(hours post fertilization).The results of qRT-PCR showed that compared with the control group,PFE could significantly increase the mRNA expression of Tyr and Tyrp2,while other groups exhibited up-regulation effects on the mRNA expression of Tyr,Tyrp1 and Tyrp2 in varying degrees.

关 键 词：补骨脂醇提物 黑色素 UPLC-Q-TOF-MS 补骨脂素 异补骨脂素 

分 类 号：R932[医药卫生—生药学]