怀山药内生真菌Chaetomium sp.的次生代谢产物研究  

Secondary metabolites from the endophytic Chaetomium sp.of Dioscorea opposita

作　　者：盛蒙珂 赵莹菲 田若丹 文春南[1,2] 刘淼 麻兵继 SHENG Meng-ke;ZHAO Ying-fei;TIAN Ruo-dan;WEN Chun-nan;LIU Miao;MA Bing-ji(College of Agronomy,Henan Agricultural University;Zhengzhou Key Laboratory of Germplasm Resources Innovation and Metabolic Engineering of Traditional Chinese Medicine,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学农学院 [2]郑州市中药材种质资源创新与代谢工程重点实验室,郑州450046

出　　处：《天然产物研究与开发》2024年第6期982-985,962,共5页Natural Product Research and Development

基　　金：国家自然科学基金(U1604169);河南省自然科学基金(232300421127);河南农业大学博士启动基金(30500662)。

摘　　要：以一株怀山药内生毛壳菌Chaetomium sp.L-45为研究对象,对其次生代谢产物进行分离和结构鉴定。对毛壳菌进行大米发酵,通过硅胶柱色谱、Sephadex LH-20、半制备高效液相色谱等分离技术分离发酵物的乙酸乙酯提取物,利用NMR、HR-ESI-MS等波谱解析技术进行化合物的结构鉴定。从目标菌株的乙酸乙酯提取物中分离得到4个化合物,分别鉴定为3,4-二甲氧基-5-羟基-6-丙基-苯甲酸(1)、5-异戊烯基-吲哚-3-羧酸(2)、lecanorin(3)和麦角甾醇(4),其中,化合物1为新化合物,化合物2和3系首次从毛壳菌属真菌中分离得到。In this study,the secondary metabolites of an endophytic Chaetomium sp.L-45 from Dioscorea opposita were isolated and identified.The ethyl acetate extract of the rice fermentation of the fungus were separated by silica gel column chromatography,Sephadex LH-20,semi-preparative High-performance liquid chromatography,etc.,and the chemical structures of the compounds were identified by NMR and HR-ESI-MS analysis.As a result,four compounds were isolated and identified as 3,4-dimethoxy-5-hydroxy-6-propyl-benzoic acid(1),5-isoprenylindole-3-carboxylic acid(2),lecanorin(3),and ergosterol(4).Among them,compound 1 is a new compound,compounds 2 and 3 were isolated from genus Chaetomium for the first time.

关 键 词：毛壳菌 次生代谢产物 提取分离 结构鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]