介绍一种骨髓活检组织脱钙方法  

Introduction of a decalcification method for bone marrow biopsy tissue

作　　者：彭先稳 缐霂 班宁溥 孙琦 Peng Xianwen;Xian Mu;Ban Ningpu;Sun Qi(State Key Laboratory of Experimental Hematology,National Clinical Research Center for Blood Diseases,Haihe Laboratory of Cell Ecosystem,Institute of Hematology&Blood Diseases Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300020,China;Tianjin Institutes of Health Science,Tianjin 301600,China)

机构地区：[1]中国医学科学院血液病医院(中国医学科学院血液学研究所),血液与健康全国重点实验室,国家血液系统疾病临床医学研究中心,细胞生态海河实验室,天津300020 [2]天津医学健康研究院,天津301600

出　　处：《中华血液学杂志》2024年第4期406-409,共4页Chinese Journal of Hematology

基　　金：中国医学科学院医学与健康科技创新工程项目(2022-I2M-1-022);国家血液系统疾病临床医学研究中心临床研究基金项目(2023NCRCA0106)。

摘　　要：骨髓活检是血液病理诊断的重要手段之一,对于多种良、恶性淋巴造血系统疾病均具有决定性的诊断意义,其诊断价值包括形态学观察,以及免疫组化、遗传学和分子生物学等辅助检测。由于骨髓活检组织本身的特殊性,脱钙是前期处理过程中必不可少的环节,其目的是将骨组织中钙盐去掉,保留完整的胶原纤维成分,以利于组织切片以及避免染色时掉片。如果骨髓活检脱钙不充分,则难以制片,但如果脱钙过度,又会严重破坏组织抗原活性,因此,一种脱钙效率高且抗原破坏小的脱钙方法对于临床病理骨髓活检十分重要。本文介绍一种高效且抗原破坏较小的骨髓活检组织脱钙方法:12%甲酸-8%盐酸脱钙液室温摇床脱钙,供广大同仁参考和指正。Bone marrow biopsy is one of the important means of hematopathological diagnosis,which has decisive diagnostic significance for various benign and malignant lymphohematopoietic system diseases.Its diagnostic value includes morphological observation,immunohistochemistry,genetics,and molecular biology testing.Owing to the unique nature of bone marrow biopsy,decalcification is an essential step in the pre-treatment process.Its purpose is to remove calcium from bone tissue,preserve intact collagen fiber components,facilitate tissue sectioning,and prevent tissue detachment during staining.If bone marrow biopsy lacks sufficient decalcification,preparing a section is difficult.Conversely,if decalcification is excessive,it can seriously disrupt tissue antigen activity.Therefore,a decalcification method with high decalcification efficiency and mild antigen damage is essential for bone marrow biopsy.This article introduces a bone marrow biopsy tissue decalcification method with high efficiency and less antigen loss:decalcification is performed at room temperature with 12%formic acid and 8%hydrochloric acid decalcification solution on a shaker.

关 键 词：骨髓活检 脱钙方法 脱钙液 抗原活性 辅助检测 病理诊断 组织切片 处理过程 

分 类 号：R36[医药卫生—病理学]