干旱胁迫下南瓜CmGolS1表达量及相关生理指标的变化  

Changes of CmGolS1 Expression and Related Physiological Indexes in Pumpkin Under Drought Stress

作　　者：吴连周 程玉琦 陈敏氡[2,3,4] 王彬 刘建汀[2,3,4] 林锦辉 叶新如 曾美娟 温庆放 朱海生[2,3,4] WU Lian-zhou;CHENG Yu-qi;CHEN Min-dong(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;Crop Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013;Fujian Key Laboratory of Vegetable Genetics and Breeding,Fuzhou,Fujian 350013;Fujian Engineering Technology Research Center for Vegetable,Fuzhou,Fujian 350013)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建省农业科学院作物研究所,福建福州350013 [3]福建省蔬菜遗传育种重点实验室,福建福州350013 [4]福建省蔬菜工程技术研究中心,福建福州350013 [5]漳州市农业技术推广站,福建漳州363000

出　　处：《安徽农业科学》2024年第12期66-70,共5页Journal of Anhui Agricultural Sciences

基　　金：福建省属公益类科研院所基本科研专项(2022R1031007);福建省农科院科技创新平台专项(CXPT2021001);福建省自然科学基金项目(2021J01494);国家大宗蔬菜产业技术体系福州综合试验站(CARS-23-G-53)。

摘　　要：[目的]通过分析干旱胁迫下“蜜本”南瓜基因CmGolS1(肌醇半乳糖苷合成酶基因1)的表达情况和可溶性糖含量的变化,解析干旱条件下南瓜的生理生化及基因水平变化,提高南瓜耐干旱能力。[方法]选用南瓜属品种‘蜜本’南瓜作为试验材料,干旱处理2~7 d。通过荧光定量PCR和可溶性糖含量测定,获得CmGolS1基因表达量的变化和细胞可溶性糖含量的变化。[结果]CmGolS1在未干旱处理的对照组几乎不表达,随着干旱处理时间延长,表达量上调,在处理第3天达到峰值,随后在干旱处理3~7 d出现一定波动,但始终相对对照组维持在高表达。对照组南瓜第一片真叶的可溶性糖含量在220μg/g左右;在干旱处理第3天开始显著上升,随后维持在320~330μg/g,可溶性糖含量在干旱处理中后期维持相对稳定。[结论]干旱胁迫下,CmGolS1上调表达,其合成产物半乳糖是多种可溶性糖的前体,可能与生理生化中可溶性糖的上调有关。可溶性糖的积累是植物抗旱过程中的一项重要生理反应,有助于降低细胞水势,抵御干旱环境。CmGolS1基因可为南瓜干旱胁迫下的生理生化变化及抗旱育种的分子机制提供重要信息。[Objective] By analyzing the expression of CmGolS1(galactinol synthase1) and the changes of soluble sugar content in ‘Miben' pumpkin under drought stress,the physiological,biochemical and gene level changes of pumpkin under drought stress were analyzed,so as to improve the drought tolerance of pumpkin.[Method] The Cucurbita cultivar ‘Miben' was selected as the research material and treated with drought for 2-7 days.The changes of CmGolS1 gene expression and soluble sugar content were detected by fluorescence quantitative PCR and soluble sugar content assay.[Result] CmGolS1 was almost not expressed in the control group,but with the progress of drought treatment,its expression level increased significantly,reached a significant high value on the third day of drought treatment,and then fluctuated within 3-7 days of drought treatment,but remained high compared with the control group.The soluble sugar content of the first true leaf of pumpkin in the control group was maintained at about 220 μg/g;it began to increase significantly at 3 days after drought treatment,and then maintained at 320-330 μg/g.The soluble sugar content remained relatively stable in the middle and late stage of drought treatment.[Conclusion] Under drought stress,the expression of CmGolS1 is up-regulated,and its synthetic product galactose is the precursor of a variety of soluble sugars,which may be related to the up regulation of soluble sugars in physiology and biochemistry.The accumulation of soluble sugar is an important physiological response in the process of plant drought resistance,which helps to improve cell water potential and resist drought environment.CmGolS1 gene can provide important information for physiological and biochemical changes of pumpkin under drought stress and molecular mechanism of drought resistance breeding.

关 键 词：南瓜 干旱 GolS 可溶性糖 

分 类 号：S642.1[农业科学—蔬菜学]