不同种植区库尔勒香梨树腐烂病菌Cytospora pyri营养体亲和性研究  

Study on Vegetative Compatibility of Cytospora pyri from Korla Fragrant Pear in Different Planting Areas

作　　者：彭莎莎 王喆 沙帅帅 张怡雯 闫成才 王兰[1] PENG Sha-sha;WANG Zhe;SHA Shuai-shuai(Agricultural College,Tarim University/Key Laboratory of Southern Xinjiang Agricultural Pest Control Corps/National and Local Joint Engineering Laboratory of High-Efficiency and High-Quality Cultivation and Deep Processing Technology of Characteristic Fruit Trees in Southern Xinjiang,Alar,Xinjiang 843300;College of Modern Agriculture,Kashgar University,Kashgar,Xinjiang 844006)

机构地区：[1]塔里木大学农学院/南疆农业有害生物综合治理兵团重点实验室/南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,新疆阿拉尔843300 [2]喀什大学现代农学院,新疆喀什844006

出　　处：《安徽农业科学》2024年第12期136-138,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然基金-新疆联合基金重点支持项目(U1903206);旱区作物逆境生物学国家重点实验室开放课题项目(CSBAA2020009);自治区研究生科研与创新项目(XJ2021G293)。

摘　　要：[目的]使用Nit突变体体系,探索来自新疆香梨种植区不同地理来源的腐烂病菌遗传多样性。[方法]首次使用Nit突变株技术(抗氯酸盐的硝酸盐利用缺陷突变体)对来自南疆3个不同种植区的13株库尔勒香梨树腐烂病菌Cytospora pyri的营养体亲和性进行了研究。[结果]13个菌株在含氯酸盐培养基(KPS)培养出115个Nit突变体,其中41个没有发生突变。在突变体稳定性鉴定中,鉴定出33个稳定的Nit突变体,82个不稳定的Nit突变体。产生的Nit突变体的表型85%被鉴定为NitD,15%被鉴定为NitB。[结论]获得2个Nit突变体,说明C.pyri的氮同化途径可能发生了2种突变。所有被检测的分离株都能够利用次黄嘌呤,使用次黄嘌呤的能力排除了钼辅助因子位点被阻断的可能性。[Objective]To use Nit mutant system to explore the genetic diversity of fragrant pear from different geographical sources in Xinjiang.[Method]In this study,Nit mutant technology(nitrate-resistant defective mutant)was used for the first time to study the nutritional affinity of Cytospora pyri,13 strains of Korla pear tree rot from three different growing areas in southern Xinjiang.[Result]115 Nit mutants were cultured in chlorate medium(KPS)from 13 strains,and 41 of them did not mutate.In the identification of mutant stability,33 stable Nit mutants and 82 unstable Nit mutants were identified.The phenotype of the resulting Nit mutants was identified as NitD 85%and NitB 15%.[Conclusion]In this study,two Nit mutants were obtained,indicating that there may be two mutations in the nitrogen assimilation pathway of C.pyri.All isolates tested were able to utilize hypoxanthine,and the ability to use hypoxanthine ruled out the possibility that molybdenum cofactor sites were blocked.

关 键 词：香梨树腐烂病菌 Cytospora pyri NIT突变体 

分 类 号：S436.612.1[农业科学—农业昆虫与害虫防治]