降解膜对高粱根际土壤微生物群落结构的影响  

Effects of biodegradable films on rhizosphere soil microbial community structure in sorghum

作　　者：刘玉兵 曹春信 黄洪明 卢华兵[4] 吕学高[4] 刘新华 Liu Yubing;Cao Chunxin;Huang Hongming;Lu Huabing;LüXuegao;Liu Xinhua(Jinhua Academy of Agricultural Sciences,Jinhua 321000,China;Key Laboratory of Innovative Utilization of Special Grain Resources in Central Zhejiang,Jinhua 321000,China;Lanxi Agricultural Technology Promotion Center,Lanxi 321100,China;Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]金华市农业科学研究院,浙江金华321000 [2]浙中特色粮作资源创新利用重点实验室,浙江金华321000 [3]兰溪市农技推广中心,浙江兰溪321100 [4]浙江省农业科学院玉米与特色旱粮研究所,浙江杭州322100

出　　处：《山西农业大学学报（自然科学版）》2024年第3期66-74,共9页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：中国农业科学院金华市院地合作项目(YDHZ2020KY04);浙江省农业重大技术协同推广计划项目(2023ZDXT03-5)。

摘　　要：[目的]本研究旨在明确降解膜对农田土壤微生物群落结构特征的影响。[方法]设置4个月降解(4J)、6个月降解(6J)、普通黑膜(PT)3种不同降解膜,以不覆膜为对照(CK),利用高通量测序技术分析土壤细菌群落结构变化,探讨降解膜覆盖农田土壤中微生物群落的变化及其对微生物生态环境效应的影响。[结果]与不覆膜处理相比,覆膜后土壤中的全氮(PT处理除外)、速效磷、速效钾以及有机质含量均得到了提升。α-蛋白细菌(Alphaproteobacteria)、放线菌(Actinobacteria)、酸杆菌Gp1(Acidobacteria_Gp1)和伽马杆菌(Gammmaproteobacteria)为双季高粱根际土壤中共同优势细菌类群。主季覆膜处理下土壤中放线菌和酸杆菌Gp1和酸杆菌Gp2(Acidobacteria_Gp2)的相对丰度均高于不覆膜的土壤;再生季覆膜处理下土壤Deltaproteobacteria相对丰度均显著高于不覆膜的土壤,且土壤细菌群落OTU(OperationalTaxonomicUnit)数大于2000,具有更高的微生物多样性。通过KEGG代谢途径分析,再生季覆膜处理下土壤中碳水化合物代谢(Carbohydrate metabolism)、氨基酸代谢(Amino acid metabolism)、辅因子和维生素代谢(Me-tabolism of cofactors and vitamins)和萜类化合物和聚酮类化合物代谢(Metabolism of terpenoids and polyketides)高于主季覆膜处理,其中以6J处理较佳。[结论]综上,6个月的降解膜(6J处理)可以有效提升土壤理化性质,改善土壤根际细菌群落生物降解,增加微生物多样性和丰富度。[Objective]The film is an important covering material for increasing crop yield and income in agricultural production.Biodegradable ground film,as a new type of soil covering material,can decompose into small molecules,eventually converting into carbon dioxide,water,and organic matter,playing an irreplaceable role in improving soil.This study aimed to clarify the influence of the biodegradable ground film on the characteristics of microbial community structure in farmland soil.[Methods]Three different degradable films were set:4-month degradation(4J),6-month degradation(6J),and common black film(PT),with non-filming field as the control(CK).The variation of soil bacterial community structure were analyzed using high-throughput sequencing technology,and the changes in microbial community and its effects on microbial ecological environment under the coverage of biodegradable films in farmland soil were explored.[Results]Compared with the non-filming treatment,the contents of total nitrogen(except for the PT treatment),available phosphorus,available potassium,and organic matter in the soil were all increased after filming.Alphaproteobacteria,Actinobacteria,Acidobacteria_Gp1 and Gammmaproteobacte-ria were the common dominant bacterial groups in the rhizosphere soil of dual-season sorghum.Under the filming treatments of the first season,the relative abundance of Actinobacteria,Acidobacteria_Gp1 and Acidobacteria_Gp2 in the soil was higher than that in the non-filming soil;Under the filming treatment of the second season,the relative abundance of Deltaproteobacte-ria in the soil was significantly higher than that in the non-filming soil,and the number of Operational Taxonomic Unit(OUT)of soil bacteria was more than 2000,showing higher microbial diversity.Through KEGG metabolic pathway analysis,soil car-bohydrate metabolism,amino acid metabolism,metabolism of cofactors and vitamins,and metabolism of terpenoids and polyketides were higher under filming treatment of the second season than that of the first season,among wh

关 键 词：生物降解膜 根际土壤 微生物群落 高通量测序 

分 类 号：S154.37[农业科学—土壤学]