病毒宏基因组学在献血人群病毒鉴定的应用研究  

Study on the application of metaviral genomics in virus identification among blood donors

作　　者：徐爱华 崔文艳 肖玮 XU Aihua;CUI Wenyan;XIAO Wei(Cangzhou Central Blood Station,Cangzhou 061001,Hebei,China)

机构地区：[1]沧州市中心血站,河北沧州061001

出　　处：《中国病原生物学杂志》2024年第6期631-635,641,共6页Journal of Pathogen Biology

基　　金：沧州市科技局重点研发计划项目(No.204106025)。

摘　　要：目的探讨宏基因组学技术在献血人群中病毒鉴定的应用潜力,并评估其在提高血液制品安全性方面的效能。方法采用前瞻性队列研究设计,收集了15名献血者的血液样本。通过QIAamp Viral RNA Mini Kit提取核酸,并使用Phusion~?High-Fidelity PCR Master Mix进行PCR扩增,随后使用NEBNext~?UltraTM DNA Library Prep Kit构建测序文库。样本通过Illumina HiSeq平台进行双端测序,再进行数据质量控制和宏基因组分析。结果在15名献血者样本中,平均总Reads数量为9.86×10^(6)±6.11×105,平均总碱基数为1.55×10^(9)±6.36×10^(7)。质量分数Q20和Q30的平均碱基数分别为1.52×10^(9)±7.04×10^(7)和1.47×10^(9)±5.95×10^(7),平均占比分别为98.81%±0.02%和95.60%±0.06%,GC含量平均占比为65.99%±0.16%。宏基因组分析鉴定出包括Dinovirus,parvovirus,torque teno virus,papillomavirus,Merkel cell polyomavirus,herpesvirus_6A,herpesvirus_6B,human pegivirus,polyomavirus在内的9类病毒。结论宏基因组学技术能够在献血样本中检测到多种病毒,包括传统筛查可能遗漏的类型。该技术可作为提高献血安全性的有效工具,有助于发现和预防病毒传播,但也需要进一步研究以优化数据处理和解释。Objective To explore the application potential of metagenomic technology in virus identification among blood donors and evaluate its effectiveness in improving the safety of blood products.Methods A prospective cohort study design was adopted,and blood samples from 15 blood donors were collected.Nucleic acids were extracted using the QIAamp Viral RNA Mini Kit and PCR amplified using Phusion High-Fidelity PCR Master Mix,followed by constructing a sequencing library using the NEBNext UltraTM DNA Library Prep Kit.The samples were subjected to paired-end sequencing on the Illumina HiSeq platform,followed by data quality control and metagenomic analysis.Results Among the 15 blood donor samples,the average number of total reads was 9.86×10^(6)±6.11×10~5,and the average number of total bases was 1.55×10^(9)±6.36×10^(7).The average base numbers of quality fractions Q20 and Q30 are 1.52×10^(9)±7.04×10^(7)and 1.47×10^(9)±5.95×10^(7)respectively,the average proportions are 98.81%±0.02%and 95.60%±0.06%respectively,and the average proportion of GC content is 65.99%±0.16%.Metagenomic analysis identified 9 types of viruses including Dinovirus,parvovirus,torque teno virus,papillomavirus,Merkel cell polyomavirus,herpesvirus_6A,herpesvirus_6B,human pegivirus,and polyomavirus.Conclusion Metagenomic techniques are capable of detecting a wide range of viruses in donated blood samples,including types that may be missed by traditional screening.The technology could serve as an effective tool to improve the safety of blood donations and help detect and prevent viral transmission,but further research is needed to optimize data processing and interpretation.

关 键 词：献血者 病毒宏基因组学 高通量测序 病原体检测 血液安全 

分 类 号：R373[医药卫生—病原生物学]